NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE80073 Query DataSets for GSE80073
Status Public on Apr 22, 2016
Title Generation of Brain Region-specific Organoids using a Miniaturized Spinning Bioreactor and Modelling ZIKV Exposure
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cerebral organoids, three-dimensional cultures that model organogenesis, provide a new platform to investigate human brain development. High cost, variability and tissue heterogeneity limit accessibility and broad applications of current organoid technologies. Here we developed a miniaturized spinning bioreactor (SpinĪ©) to generate forebrain-specific organoids from human iPSCs. These organoids recapitulate key features of human cortical development, including progenitor zone organization, neurogenesis, gene expression, and importantly, a distinct human-specific outer radial glia cell layer. We have also developed protocols to generate midbrain and hypothalamic organoids. Finally, we employed this forebrain organoid platform to model Zika virus (ZIKV) exposure. Quantitative analyses revealed that preferential, productive ZIKA infection of cortical neural progenitors leads to increased cell death and reduced proliferation, resulting in decreased neuronal cell layer volume that resembles microcephaly. Together, our brain region-specific organoids and SpinĪ© provide an accessible and versatile platform for modeling human brain development and diseases, and for compound testing.
 
Overall design Time course of human cerebral organoid cultures. No Zika virus infection is involved.
 
Contributor(s) Yao B
Citation(s) 27118425
Submission date Apr 08, 2016
Last update date May 15, 2019
Contact name Bing Yao
E-mail(s) bing.yao@emory.edu
Phone 4047271725
Organization name Emory University
Department Human Genetics
Lab Bing Yao Lab
Street address 615 Michael St. Rm 323
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (20)
GSM2112653 C12-d26-1.1
GSM2112654 C12-d26-1.2
GSM2112655 C12-d26-2.1
Relations
BioProject PRJNA317792
SRA SRP073044

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80073_Organoid.gene_exp.txt.gz 4.9 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap