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| Status |
Public on Mar 22, 2016 |
| Title |
Genome-wide mapping of Hif-1α binding sites in zebrafish [microarray] |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of gene expression changes in the von Hippel Lindau when mutant compared to wild-type at 4dpf using a whole genome microarray expression profiling. The von Hippel Lindau mutant displays a systemic hypoxic response under normoxic conditions. We performed single-colour microarrays to identify the gene expression changes which underpin the hypoxic phenotype. We used 3 biological replicates from both mutant and control, followed by analysis using Limma to identify significant gene expression changes. This work, together with ChIP-seq data for the Hif-1α in von Hippel Lindau mutants, should allow for the Hif-1α dependency of these gene expression changes to be assessed.
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| Overall design |
The changes in gene expression between von Hippel Lindau mutants and wild-type controls was measured at 4 days post fertilisation. For both wild-type and mutant, 3 biological replicates, each of 30 embryos were used.
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| Contributor(s) |
Greenald D, Jeyakani J, Pelster B, Sealy I, Mathavan S, van Eeden FJ |
| Citation(s) |
26559940 |
| Submission date |
Jul 14, 2015 |
| Last update date |
Jun 21, 2016 |
| Contact name |
Justin Jeyakani Joseph Gnanakkan |
| E-mail(s) |
gnanakkan@gis.a-star.edu.sg
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| Phone |
68088173
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| Organization name |
Genome Institute of Singapore
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| Department |
Computational and Systems Biology
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| Street address |
60, Biopolis street
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| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE70886 |
Genome-wide mapping of Hif-1α binding sites in zebrafish |
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| Relations |
| BioProject |
PRJNA289856 |
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