 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 01, 2015 |
| Title |
Regulation of protein translation during mitosis |
| Organism |
Homo sapiens |
| Experiment type |
Other
Expression profiling by high throughput sequencing
|
| Summary |
Entry into and exit from mitosis is driven by precisely-timed changes in protein abundance, and involves transcriptional regulation and protein degradation. However, the role of translational regulation in modulating cellular protein content during mitosis remains poorly understood. Here, using ribosome profiling, we show that translational, rather than transcriptional regulation is the dominant mechanism for modulating protein synthesis at mitotic entry. The vast majority of regulated mRNAs are translationally repressed, which contrasts previous findings of selective mRNA translational activation at mitotic entry. One of the most pronounced translationally repressed genes in mitosis is Emi1, an inhibitor of the anaphase promoting complex (APC), which is degraded during mitosis. We show that Emi1 degradation is insufficient for full APC activation and that simultaneous translational repression is required. These results provide a genome-wide view of protein translation during mitosis and suggest that translational repression may be used to ensure complete protein inactivation
|
| |
|
| Overall design |
Ribosome profiling and mRNA-seq from 3 time points in the cell cycle
|
| |
|
| Contributor(s) |
Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD |
| Citation(s) |
26305499 |
| Submission date |
Apr 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Marvin Tanenbaum |
| E-mail(s) |
marvin.tanenbaum@ucsf.edu
|
| Organization name |
UCSF
|
| Department |
CMP
|
| Lab |
Ron Vale
|
| Street address |
Genentech Hall, MC 2200, Room N316, 600 16th street
|
| City |
San Francisco |
| State/province |
ca |
| ZIP/Postal code |
94158-2517 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (12)
|
| GSM1657720 |
Ribosome footprints from G1 cells, pretreated with cycloheximide_1 |
| GSM1657721 |
Ribosome footprints from G1 cells, pretreated with cycloheximide_2 |
| GSM1657722 |
Ribosome footprints from G2 cells, pretreated with cycloheximide_1 |
| GSM1657723 |
Ribosome footprints from G2 cells, pretreated with cycloheximide_2 |
| GSM1657724 |
Ribosome footprints from M cells, pretreated with cycloheximide_1 |
| GSM1657725 |
Ribosome footprints from M cells, pretreated with cycloheximide_2 |
| GSM1657726 |
mRNA-seq from G1 cells, pretreated with cycloheximide_1 |
| GSM1657727 |
mRNA-seq from G1 cells, pretreated with cycloheximide_2 |
| GSM1657728 |
mRNA-seq from G2 cells, pretreated with cycloheximide_1 |
| GSM1657729 |
mRNA-seq from G2 cells, pretreated with cycloheximide_2 |
| GSM1657730 |
mRNA-seq from M cells, pretreated with cycloheximide_1 |
| GSM1657731 |
mRNA-seq from M cells, pretreated with cycloheximide_2 |
|
| Relations |
| BioProject |
PRJNA281177 |
| SRA |
SRP057253 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67902_Supplemental_table_S1_mRNA_analysis.txt.gz |
847.2 Kb |
(ftp)(http) |
TXT |
| GSE67902_Supplemental_table_S2_TE_analysis.txt.gz |
589.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...