|
| Status |
Public on Apr 11, 2016 |
| Title |
ChIP-seq of erythroid progenitors treated with vehicle or the HDAC1/2-selective inhibitor ACY-957 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Establish the DNA binding profiles of HDAC1, HDAC2 and Gata2 in erythroid progenitors derived from human CD34+ bone marrow cells. Determine the effects of HDAC1/2 inhibition on the DNA binding profiles of Gata2.
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| |
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| Overall design |
5 samples in total. Cells have either been treated with vehicle or ACY-957 (an HDAC1/2 selective inhibitor) prior to immunoprecipitation. Sample 1 is chromatin from vehicle treated cells immunoprecipitated with anti-HDAC1 antibody. Sample 2 is chromatin from vehicle treated cells immunoprecipitated with anti-HDAC2 antibody. Sample 3 and 4 are chromatin from vehicle treated or ACY-957 treated cells immunoprecipitated with anti-Gata2, respectively. Sample 5 is the input sample, which is a mix of non-immunoprecipitated chromatin from vehicle treated and ACY-957 treated cells.
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| Contributor(s) |
Shearstone JR, Golonzhka O, Chonkar A, Tamang D, van Duzer JH, Jones SS, Jarpe MB |
| Citation(s) |
27073918 |
| Submission date |
Aug 26, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeff Shearstone |
| E-mail(s) |
jshearstone@acetylon.com
|
| Phone |
617-245-1321
|
| Organization name |
Acetylon Pharmaceuticals
|
| Department |
Biology
|
| Street address |
70 Fargo Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02210 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE60793 |
Chemical inhibition of Histone Deacetylases 1 and 2 induces fetal hemoglobin through activation of Gata2 |
|
| Relations |
| BioProject |
PRJNA259552 |
| SRA |
SRP045797 |
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