|
| Status |
Public on May 09, 2014 |
| Title |
Identification of targets regulated by SELP in HSC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand the underlying mechanism by which Alox15 gene is required by HSCs, we performed a comparative DNA microarray analysis using total RNA isolated from wild type Lin-Sca-1+c-Kit+, SELP-/- Lin-Sca-1+c-Kit+. The result was validated by quantitative real-time PCR analysis of wild type Lin-Sca-1+c-Kit+ and SELP-/- Lin-Sca-1+c-Kit+.
Cancer stem cells are responsible for the initiation and maintenance of some types of cancer, and few effective target genes in these stem cells have been identified. Here we show that the selp is essential for the survival of leukemia stem cells (LSCs) in BCR-ABL-induced chronic myeloid leukemia (CML). To understand the underlying mechanism through which SELP regulates the function of HSCs, the gene expression profiles between WT and Selp-/- HSCs were compared.
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| Overall design |
To understand the underlying mechanism through which SELP regulates the function of HSCs, the gene expression profiles between WT and Selp-/- HSCs were compared.
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| |
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| Contributor(s) |
Chen Y, Shan Y, Li S |
| Citation(s) |
25105362 |
| Submission date |
May 08, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
yaoyu chen |
| E-mail(s) |
chenyaoyumaine@gmail.com
|
| Phone |
7742220688
|
| Organization name |
NIBR
|
| Department |
ONC
|
| Street address |
250 Mass ave
|
| City |
cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
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| Relations |
| BioProject |
PRJNA246487 |
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