 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 31, 2014 |
| Title |
Suberin degradation by Aspergillus nidulans |
| Platform organisms |
Aspergillus nidulans FGSC A4; Neurospora crassa OR74A |
| Sample organism |
Aspergillus nidulans |
| Experiment type |
Expression profiling by array
|
| Summary |
The study aims essentially at the characterisation of suberin degradation mechanisms by Aspergillus nidulans, at a fundamental level. Suberin is an important protective barrier in plant, thus the study of its biodegradation significantly impacts on phytopatology. In addition, fungal suberin degrading enzymes might provide important insights to develop new waste management, bioremediation and biodeterioration prevention strategies.
|
| |
|
| Overall design |
The Aspergillus nidulans cultures were grown for two days in mineral media containing glucose (T0). After this time point, the extracellular media was replaced by fresh mineral media containing suberin as the sole carbon source and allowed to incubate for two (T1), four (T2) and six (T3) days. To investigate the mechanisms by which the fungus was able to degrade suberin, the fungal transcriptome (three biological replicates) was analysed using Affymetrix microarrays. Pair-wise comparisons were used to identify the set of genes with altered expression levels between the control (grown on glucose) and during growth on suberin for two, four or six days.
|
| |
|
| Contributor(s) |
Pereira CS, Becker JD, Alves PC, Martins I, Hartmann DO, Garcia H, He J |
| Citation(s) |
25043916 |
| Submission date |
Jan 27, 2014 |
| Last update date |
Oct 31, 2014 |
| Contact name |
Cristina Silva Pereira |
| E-mail(s) |
spereira@itqb.unl.pt
|
| Organization name |
Instituto de Tecnologia Química e Biológica (ITQB)
|
| Lab |
Applied and Environmental Mycology Laboratory (AEM)
|
| Street address |
Rua da Quinta Grande nº6
|
| City |
Lisboa |
| ZIP/Postal code |
2780-156 Oeiras |
| Country |
Portugal |
| |
|
| Platforms (1) |
| GPL18227 |
FungiANC (GeneChip FungiANCa520710F) |
|
| Samples (12)
|
| GSM1314873 |
Aspergillus nidulans grown in glucose media for two days, biological rep. 1 |
| GSM1314874 |
Aspergillus nidulans grown in glucose media for two days, biological rep. 2 |
| GSM1314875 |
Aspergillus nidulans grown in glucose media for two days, biological rep. 3 |
| GSM1314876 |
Aspergillus nidulans grown in suberin media, for two days, biological rep. 1 |
| GSM1314877 |
Aspergillus nidulans grown in suberin media, for two days, biological rep. 2 |
| GSM1314878 |
Aspergillus nidulans grown in suberin media, for two days, biological rep. 3 |
| GSM1314879 |
Aspergillus nidulans grown in suberin media, for four days, biological rep. 1 |
| GSM1314880 |
Aspergillus nidulans grown in suberin media, for four days, biological rep. 2 |
| GSM1314881 |
Aspergillus nidulans grown in suberin media, for four days, biological rep. 3 |
| GSM1314882 |
Aspergillus nidulans grown in suberin media, for six days, biological rep. 1 |
| GSM1314883 |
Aspergillus nidulans grown in suberin media, for six days, biological rep. 2 |
| GSM1314884 |
Aspergillus nidulans grown in suberin media, for six days, biological rep. 3 |
|
| Relations |
| BioProject |
PRJNA236608 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54427_RAW.tar |
15.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...