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Status |
Public on Jul 03, 2014 |
Title |
C. Elegans expression: toxic vs. adequate vs. low selenium |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
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Summary |
Genome-wide expression analysis in C. Elegans grown in axenic media with low to toxic selenium concentrations We performed Affymetrix micorarray-based transcriptional profiling on wild-type C. Elegans Bristol N2 grown in low Se axenic media supplemented with five concentrations of selenium, from low to toxic, and harvested at the L4-larva stage.
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Overall design |
RNA was prepared for hybridization to Affy microarrays from synchronized cultures of wild-type C. elegans seeded in low Se axenic media, supplemented with graded 0, 0.05, 0.1, 0.2, and 0.4 mM Se added as sodium selenite, and harvested at the L4-larva stage (1 culture/sample per Se concentgration).
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Contributor(s) |
Sunde RA |
Citation(s) |
24971995 |
Submission date |
Jan 13, 2014 |
Last update date |
Jul 06, 2016 |
Contact name |
Roger A Sunde |
E-mail(s) |
sunde@nutrisci.wisc.edu
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Phone |
608-262-4044
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Organization name |
University of Wisconsin
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Department |
Nutritional Sciences
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Street address |
1415 Linden Dr
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706-1527 |
Country |
USA |
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Platforms (1) |
GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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Samples (5)
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Relations |
BioProject |
PRJNA234349 |