 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 28, 2014 |
| Title |
BAF155 methylation at R1064 creates unique chromatin association patterns |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
CARM1, a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drastic phenotypic and biochemical changes. The CARM1 KO cell lines enabled identification of novel CARM1 substrates, notably the SWI/SNF core subunit BAF155. Methylation of BAF155 at R1064 was found to be an independent prognostic biomarker for cancer recurrence and to regulate breast cancer cell migration and metastasis. Further, CARM1-mediated BAF155 methylation affects gene expression by directing methylated BAF155 to unique chromatin regions (e.g., c-Myc pathway genes). Collectively our studies uncover a mechanism by which BAF155 acquires tumorigenic functions via arginine methylation.
|
| |
|
| Overall design |
Examination of methylation of BAF155 (R1064) in breast cancer cells
|
| |
|
| Contributor(s) |
Wang L, Meyer M, Pike JW, Xu W |
| Citation(s) |
24434208 |
| Submission date |
Dec 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Lu Wang |
| E-mail(s) |
wanglunju@gmail.com
|
| Organization name |
UW madison
|
| Department |
Oncology
|
| Street address |
1400 university ave
|
| City |
madison |
| State/province |
wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA230559 |
| SRA |
SRP033492 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52964_RAW.tar |
430.4 Mb |
(http)(custom) |
TAR (of BED, BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...