NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE43043 Query DataSets for GSE43043
Status Public on May 16, 2013
Title Effect of temperature and irradiation intensity on grapevine berry ripening in Muscat Hamburg fruiting cuttings
Organism Vitis vinifera
Experiment type Expression profiling by array
Summary At the onset of ripening (veraison), Vitis vinifera L. cv Muscat Hamburg fruiting cuttings were exposed to four different environmental regimes in two growth chambers set up: 20/15ºC (day/night) and 30/25ºC (day/night) and with two different photosynthetic photon flux density (PPFD) conditions (200 and 400 μmol•m−2•s−1 PPFD) at grape bunch level during a 14 h photoperiod. Fruiting cuttings were selected to have similar grape bunch size avoiding changes in berry quality. At least 9 plants were transferred to each treatment. Global pericarp gene expression was compared among the four growth conditions using the GrapeGen GeneChip®. The transcriptomic analysis was carried out in 14 DAT berries in order to identify stable responses to differential temperature and PPFD conditions not masked by quick transient responses to the environmental change. Moreover, grapes of the same density range (100-110 g NaCl·L-1)) from all four treatments were compared to ensure that treatment effects other than ripening rate shifts originated by the treatments, at least in terms of saccharimetric ripening, were being studied. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Pablo Carbonell-Bejerano. The equivalent experiment is VV46 at PLEXdb.]
 
Overall design temperature: 30/25 C - light: 400 μmol·m−2·s−1(3-replications); temperature: 30/25 C - light: 200 μmol·m−2·s−1(3-replications); temperature: 20/15 C - light: 400 μmol·m−2·s−1(3-replications); temperature: 20/15 C - light: 200 μmol·m−2·s−1(3-replications)
Web link http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=VV46
 
Contributor(s) Carbonell-Bejerano P, Martínez-Zapater JM
Citation(s) 23659918
Submission date Dec 19, 2012
Last update date May 18, 2013
Contact name PLEXdb Curator, Sudhansu Dash
Organization name Iowa State University
Department Virtual Reality Application Center
Lab PLEXdb
Street address 1023 Crop Genomes Informatics Lab (Iowa State University)
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL11004 Affymetrix GrapeGen Vitis vinifera Microarray [CDF: GrapeGena520510F]
Samples (12)
GSM1055569 temperature: 30/25 C - light: 400 μmol·m−2·s−1 - rep1
GSM1055570 temperature: 30/25 C - light: 400 μmol·m−2·s−1 - rep2
GSM1055571 temperature: 30/25 C - light: 400 μmol·m−2·s−1 - rep3
Relations
BioProject PRJNA184175

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43043_RAW.tar 28.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap