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| Status |
Public on Dec 04, 2012 |
| Title |
Stress response in the yajL (DJ-1 homolog) mutant of Escherichia coli |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
YajL is the most closely related Escherichia coli homolog of Parkinsonism-associated protein DJ-1, a protein with a yet undefined function in the oxidative stress response. YajL protects cells against oxidative stress-induced protein aggregation and functions as a covalent chaperone for the thiol proteome, including FeS proteins. To clarify the cellular responses to YajL deficiency, transcriptional profiling of the yajL mutant was performed. As compared to the parental strain, the yajL mutant overexpressed genes coding for chaperones, proteases, chemical chaperone transporters, superoxide dismutases, catalases, peroxidases, components of thioredoxin and glutaredoxin systems, iron transporters, ferritins and FeS cluster biogenesis enzymes, DNA-repair proteins, RNA chaperones and small regulatory RNAs. It also overexpressed the RNA polymerase stress sigma factors sigma S (multiple stresses) and sigma 32 (protein stress) and activated the OxyR and SoxRS oxidative stress transcriptional regulators, which together trigger the global stress response. The yajL mutant also overexpressed genes involved in septation and adopted a shorter and rounder shape characteristic of stressed bacteria. Biochemical experiments showed that this upregulation of many stress genes resulted in increased expression of stress proteins and improved biochemical function. Thus, protein defects resulting from the yajL mutation trigger the onset of a robust and global stress response in a prokaryotic model of DJ-1-associated Parkinsonism.
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| Overall design |
We performed microarray analysis of the transcriptome response of the yajL mutant and its parental strain in the exponential phase of growth (grown in aerobiosis in LB medium to OD600 = 0.3) in the absence of any exogenous stress. Two replicates per strain (wild type, yajL mutant).
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| Contributor(s) |
Richarme G |
| Citation(s) |
23292772 |
| Submission date |
Dec 03, 2012 |
| Last update date |
Mar 08, 2019 |
| Contact name |
gilbert richarme |
| E-mail(s) |
richarme@paris7.jussieu.fr
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| Organization name |
Institut Jacques Monod
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| Lab |
stress molecules
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| Street address |
15 rue helene brion
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| City |
paris |
| State/province |
ile de france |
| ZIP/Postal code |
75013 |
| Country |
France |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (4)
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| GSM1048193 |
Intensity measures in wild type strain, replicate 1 |
| GSM1048194 |
Intensity measures in yajL mutant strain, replicate 1 |
| GSM1048195 |
Intensity measures in wild type strain, replicate 2 |
| GSM1048196 |
Intensity measures in yajL mutant strain, replicate 2 |
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| Relations |
| BioProject |
PRJNA182760 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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