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Status |
Public on Jul 12, 2012 |
Title |
High-thoughput Illumina RNA sequencing to identify downstream target genes of RABBIT EARS (RBE) in the flowers of Arabidopsis thaliana |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In order to identify putative downstream target genes of RBE, we sequenced mRNA from dexamethasone (DEX) and mock treated transgenic Arabidopsis line 35S:GR-RBE (RBE coding region fused to a glucocorticoid receptor domain driven by the constitutive 35S promoter) floral tissues. We compared the results from DEX and mock treatments and focused on the 832 genes whose expression was significantly reduced (P < 0.025) by 2-fold or more in DEX as compared to mock-treated plants. In this analysis, we identified MIR164c (EEP1) as a candidate target of RBE, which was further confirmed by other molecular and genetic analyses. Regulation of MIR164c by RBE is important for normal floral organ formation in Arabidopsis.
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Overall design |
We used two biological replicates, each with two technical replicates for four hour DEX or mock treated floral tissues to produce 8 sequencing libraries.
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Contributor(s) |
Huang T, López-Giráldez F, Townsend JP, Irish VF |
Citation(s) |
22573623 |
Submission date |
Mar 13, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Francesc Lopez |
E-mail(s) |
francesc.lopez@yale.edu
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Organization name |
Yale University
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Department |
Department of Genetics
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Lab |
YCGA
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Street address |
P.O. Box 27386
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City |
West Haven |
State/province |
CT |
ZIP/Postal code |
06516 |
Country |
USA |
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Platforms (1) |
GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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Samples (8)
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GSM894355 |
mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 1, techinical replicate 1 |
GSM894356 |
mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 1, techinical replicate 2 |
GSM894357 |
mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 2, techinical replicate 1 |
GSM894358 |
mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 2, techinical replicate 2 |
GSM894359 |
mRNA from mock-treated 35S:GR-RBE floral buds, biological replicate 1, techinical replicate 1 |
GSM894360 |
mRNA from mock-treated 35S:GR-RBE floral buds, biological replicate 1, techinical replicate 2 |
GSM894361 |
mRNA from mock-treated 35S:GR-RBE floral buds, biological replicate 2, techinical replicate 1 |
GSM894362 |
mRNA from mock-treated 35S:GR-RBE floral buds, biological replicate 2, techinical replicate 2 |
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Relations |
SRA |
SRP011435 |
BioProject |
PRJNA153493 |
Supplementary file |
Size |
Download |
File type/resource |
GSE36469_LOX_output_combined_final.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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