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Series GSE36469 Query DataSets for GSE36469
Status Public on Jul 12, 2012
Title High-thoughput Illumina RNA sequencing to identify downstream target genes of RABBIT EARS (RBE) in the flowers of Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary In order to identify putative downstream target genes of RBE, we sequenced mRNA from dexamethasone (DEX) and mock treated transgenic Arabidopsis line 35S:GR-RBE (RBE coding region fused to a glucocorticoid receptor domain driven by the constitutive 35S promoter) floral tissues. We compared the results from DEX and mock treatments and focused on the 832 genes whose expression was significantly reduced (P < 0.025) by 2-fold or more in DEX as compared to mock-treated plants. In this analysis, we identified MIR164c (EEP1) as a candidate target of RBE, which was further confirmed by other molecular and genetic analyses. Regulation of MIR164c by RBE is important for normal floral organ formation in Arabidopsis.
 
Overall design We used two biological replicates, each with two technical replicates for four hour DEX or mock treated floral tissues to produce 8 sequencing libraries.
 
Contributor(s) Huang T, López-Giráldez F, Townsend JP, Irish VF
Citation(s) 22573623
Submission date Mar 13, 2012
Last update date May 15, 2019
Contact name Francesc Lopez
E-mail(s) francesc.lopez@yale.edu
Organization name Yale University
Department Department of Genetics
Lab YCGA
Street address P.O. Box 27386
City West Haven
State/province CT
ZIP/Postal code 06516
Country USA
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (8)
GSM894355 mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 1, techinical replicate 1
GSM894356 mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 1, techinical replicate 2
GSM894357 mRNA from DEX-treated 35S:GR-RBE floral buds, biological replicate 2, techinical replicate 1
Relations
SRA SRP011435
BioProject PRJNA153493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36469_LOX_output_combined_final.txt.gz 1.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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