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| Status |
Public on Aug 10, 2012 |
| Title |
Transcriptome analysis of the uninjured Muller glia (MG) and MG derived retinal progenitors at 4 day post injured zebrafish retina |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
The study was aimed at comparing the transcriptome of MG cells of the retina with the progenitors derived from them after an injury. This information will help in the identification of factors that are responsible for the retinal regeneration.
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| Overall design |
Muller glia were isolated by fluorescent activated cell sorting (FACS) using uninjured retinas from transgenice zebrafish (gfap:gfp) where green florescent protein (GFP) is under the control of the glial fibrillary acidic protein (gfap) promoter. MG-derived retinal progenitors were isolated by FACS at 4 days post retinal injury from 1016 tuba1a:gfp transgenic fish where GFP is driven by the tuba1a promoter which is specifically activated in these progenitors. Total RNA was isolated from these cell populations and subjected to microarray analysis to compare their transcriptomes. Samples were prepared in duplicate.
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| Contributor(s) |
Ramachandran R, Goldman D |
| Citation(s) |
23000964 |
| Submission date |
Mar 01, 2012 |
| Last update date |
Oct 17, 2012 |
| Contact name |
Rajesh Ramachandran |
| E-mail(s) |
rajeshra@umich.edu
|
| Phone |
17349363649
|
| Organization name |
University of Michigan
|
| Department |
Molecular and Behavioral Neuroscience Institute
|
| Lab |
Goldman Lab
|
| Street address |
109 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL7302 |
Agilent-015064 D. rerio (Zebrafish) Oligo Microarray 4x44K G2519F (Probe Name version) |
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| Samples (4)
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| GSM883421 |
Muller glia_uninjured_0 days post injury_rep1 |
| GSM883422 |
Muller glia_uninjured_0 days post injury_rep2 |
| GSM883423 |
Muller glia derived progenitors_4 day post injury_rep1 |
| GSM883424 |
Muller glia derived progenitors_4 day post injury_rep2 |
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| Relations |
| BioProject |
PRJNA152863 |
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