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| Status |
Public on May 31, 2012 |
| Title |
Multiple changes at the mucosal surface are induced by protective SIV vaccination |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by array
|
| Summary |
Systemic vaccination with the attenuated virus SIVmac239-∆Nef provides sterilizing or partial protection to rhesus monkeys challenged with WT SIV strains, providing important opportunities to study key immunological components of a protective host response. Here we show that intravenous vaccination with SIVmac239-∆Nef provides two potentially crucial immunological barriers localized at mucosal surfaces that correlate with the vaccine’s protective effects against WT SIVmac251 vaginal challenge: 1) a conditioned and coordinated response from the mucosal epithelium that blunts the early inflammatory and chemotactic signalling cascade that aids virus propagation and expansion; 2) early on-site generation/diversification of SIV-specific Abs from ectopic germinal center-like lymphoid aggregates. This unique host response to WT SIVmac251 in the female reproductive tract of SIVmac239-∆Nef-vaccinated animals points to a multi-layered strategy for a protective host response during immunodeficiency virus exposure—rapid induction of humroal immunity at mucosal surfaces without the deleterious inflammatory side effects tied to innate recognition of virus. This vaccine-induced host response highlights potential key protective mechanisms needed for an effective HIV vaccine
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| Overall design |
Total RNA was isolated from the cervix of 17 Indian Rhesus macaques (3 uninfected animals; 5 unvaccinated animals 4-5 days post vaginal exposure with SIVmac251; 4 SIVmac239-∆Nef-vaccinated animals before challenge; 5 SIVmac239-∆Nef-vaccinated animals 4-5 days post vaginal exposure with SIVmac251) and prepared for hybridization on Affymetrix GeneChip Rhesus Macaque Genome Arrays. Replicate arrays were performed for a number of the samples to minimize assay noise and significant host genes altered during virus exposure in female reproductive tract tissue were identified by their associated q-values (< 0.2) and fold change in expression (> 1.2).
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| Contributor(s) |
Smith AJ, Wietgrefe SW, Reilly CS, Duan L, Shang L, Li Q, Johnson PR, Haase AT |
| Citation(s) |
25143442 |
| Submission date |
Oct 26, 2011 |
| Last update date |
Oct 01, 2014 |
| Contact name |
Anthony James Smith |
| E-mail(s) |
smit4263@umn.edu
|
| Phone |
612-624-9130
|
| Fax |
612-626-0623
|
| Organization name |
University of Minnesota
|
| Department |
Microbiology
|
| Lab |
Ashley T. Haase
|
| Street address |
420 Delaware Street S.E.
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL3535 |
[Rhesus] Affymetrix Rhesus Macaque Genome Array |
|
| Samples (17)
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| Relations |
| BioProject |
PRJNA149033 |
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