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| Status |
Public on Oct 05, 2011 |
| Title |
Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri |
| Organism |
Ictalurus punctatus |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine the global gene transcriptional changes in vaccinated channel catfish anterior kidney after immersion challenge with a virulent Edwardsiella ictaluri compared to sham vaccinated catfish control.
The attenuated Edwardsiella ictaluri used for vaccination was Aquavac-ESC which was commercially available, the virulent strain of Edwardsiella ictaluri is the AL93-58 strain.
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| Overall design |
A six chip study using total RNA samples including 3 controls (no vaccination, no challenge) and 3 treated groups (challenge after vaccination). Each RNA sample used for microarray was pooled fish anterior kidney samples (5 fish anterior kidney samples were pooled as one). Each chip measrues the expression level of 65,182 genes. For each sequence, 3 probes were selected. Two copies were prepared on arrays.
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| Contributor(s) |
Pridgeon JW, Yeh H, Shoemaker CA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 04, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
julia pridgeon |
| E-mail(s) |
julia.pridgeon@ars.usda.gov
|
| Phone |
3348873741
|
| Fax |
3348872983
|
| Organization name |
USDA, ARS
|
| Department |
AAHRU
|
| Street address |
990 WIRE ROAD
|
| City |
AUBURN |
| State/province |
AL |
| ZIP/Postal code |
36832 |
| Country |
USA |
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| Platforms (1) |
| GPL14562 |
AAHRU_Channel catfish_65K array |
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| Samples (6)
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| GSM794590 |
sham vaccinated fish RNA sample_control_rep1 |
| GSM794591 |
sham vaccinated fish RNA sample_control_rep2 |
| GSM794592 |
sham vaccinated fish RNA sample_control_rep3 |
| GSM807738 |
vaccinated fish followed by challenge RNA sample_challenge_rep1 |
| GSM807739 |
vaccinated fish followed by challenge RNA sample_challenge_rep2 |
| GSM807740 |
vaccinated fish followed by challenge RNA sample_challenge_rep3 |
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| Relations |
| BioProject |
PRJNA147087 |
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