 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 01, 2011 |
| Title |
Evidence for widespread changes in promoter methylation profile in human placenta in response to increasing gestational age and environmental/stochastic factors. |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
Background: the human placenta facilitates the exchange of nutrients, gas and waste between the fetal and maternal circulations. It also protects the fetus from the maternal immune response. Due to its role at the feto-maternal interface, the placenta is subject to many environmental exposures that can potentially alter its epigenetic profile. Previous studies have reported gene expression differences in placenta over gestation, as well as inter-individual variation in expression of some genes. However, the factors contributing to this variation in gene expression remain poorly understood. Results: in this study, we performed a genome-wide DNA methylation analysis of gene promoters in placenta tissue from three pregnancy trimesters. We identified large-scale differences in global DNA methylation levels between first, second and third trimesters, with an overall progressive increase in average methylation from first to third trimester. The most differentially methylated genes included many immune regulators, reflecting the change in placental immuno-modulation as pregnancy progresses. We also detected increased inter-individual variation in the third trimester relative to first and second, supporting an accumulation of environmentally induced (or stochastic) changes in DNA methylation pattern. These highly variable genes were enriched for those involved in amino acid and other metabolic pathways, potentially reflecting the adaptation of the human placenta to different environments. Conclusions : the identification of cellular pathways subject to drift in response to environmental influences provide a basis for future studies examining the role of specific environmental factors on DNA methylation pattern and placenta-associated adverse pregnancy outcomes.
|
| |
|
| Overall design |
A total of 42 samples, with 18 first trimester, 10 second trimester, 14 full term placenta
|
| |
|
| Contributor(s) |
Novakovic B, Yuen RK, Gordon L, Panaherrera MS, Sharkey A, Moffett A, Craig JM, PRobinson W, Saffery R |
| Citation(s) |
22032438 |
| Submission date |
Aug 31, 2011 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Boris Novakovic |
| E-mail(s) |
boris.novakovic@mcri.edu.au
|
| Phone |
0434980073
|
| Organization name |
Murdoch Children's Research Institute
|
| Street address |
Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
|
| Samples (42)
|
|
| Relations |
| BioProject |
PRJNA145291 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31781_RAW.tar |
5.8 Mb |
(http)(custom) |
TAR |
| GSE31781_matrix_signal_intensities.txt.gz |
5.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...