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| Status |
Public on Jun 18, 2012 |
| Title |
Nucleosome occupancy dynamics in yeast BY4741 responding to 0.4mM H2O2 over time (0-60 min) |
| Organism |
Saccharomyces cerevisiae S288C |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Numerous factors have been implicated in regulating gene expression changes, including changes to nucleosome occupancy. Here we followed dynamic changes to nucleosome occupancy, gene expression and DNA binding of the transcription factor Msn2p genome-wide in yeast cells responding to hydrogen peroxide to reveal new relationships between regulators of stress-dependent gene expression.
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| Overall design |
Nucleosome occupancy was measured by MNase-Seq in response to .4mM H2O2 in the S288c yeast derivative BY4741. A single replicate was performed for the time-course experiment with time points at 0, 4, 12, 20, 40 and 60 minutes after treatment.
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| Contributor(s) |
Huebert DJ, Gasch AP |
| Citation(s) |
22354995 |
| Submission date |
Jul 25, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Dana J Huebert |
| Organization name |
University of Wisconsin-Madison
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| Department |
Genetics
|
| Lab |
Audrey Gasch
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| Street address |
425G Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL13965 |
Illumina Genome Analyzer II (Saccharomyces cerevisiae S288c) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE30901 |
Dynamic changes to nucleosome occupancy and genomic expression in yeast responding to oxidative stress |
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| Relations |
| SRA |
SRP007842 |
| BioProject |
PRJNA154583 |
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