|
| Status |
Public on Jul 16, 2011 |
| Title |
Pseudomonas aeruginosa gene expression in artificial sputum medium using a broad capture array designated the PANarray |
| Platform organisms |
Pseudomonas aeruginosa UCBPP-PA14; Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa 2192; Pseudomonas aeruginosa C3719; Pseudomonas aeruginosa PA7; Pseudomonas aeruginosa PACS2; Pseudomonas aeruginosa LESB58; Pseudomonas aeruginosa AES-1R |
| Sample organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
The PANarray design (GPL13324) contains the genes of eight P. aeruginosa genomes in non-redundant format, thus allowing identification of expression of non-PAO1 and other P. aeruginosa genes.
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| Overall design |
For the series GSE28152, isogenic isolates were sequentially collected from two cystic fibrosis (CF) patients several years apart. The isolates had not been eradicated in the meantime and represent persister strains. One was an Australian Epidemic Strain-1 isolate and the other a non-epidemic strain. Strains were cultured in an artificial sputum medium (ASMDM) closely resembling CF sputum.
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| Contributor(s) |
Manos J, Seemann T, Whitchurch C, Parker D, Naughton S, Turnbull L, Rose B |
| Citation(s) |
21935417 |
| Submission date |
Mar 24, 2011 |
| Last update date |
Nov 24, 2015 |
| Contact name |
Jim Manos |
| E-mail(s) |
jim.manos@sydney.edu.au
|
| Phone |
+612 9351 8942
|
| Organization name |
University of Sydney
|
| Street address |
Camperdown
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL13324 |
University of Sydney CombiMatrix 12K PANarray |
|
| Samples (8)
|
| GSM697393 |
P. aeruginosa early infection isolate AES-1R replicate 1 |
| GSM697394 |
P. aeruginosa early infection isolate AES-1R replicate 2 |
| GSM697395 |
P. aeruginosa chronic infection isolate AES-1M replicate 1 |
| GSM697396 |
P. aeruginosa chronic infection isolate AES-1M replicate 2 |
| GSM697397 |
P. aeruginosa early infection isolate 1Mel replicate 1 |
| GSM697398 |
P. aeruginosa early infection isolate 1Mel replicate 2 |
| GSM697399 |
P. aeruginosa intermittent infection isolate 5Mel replicate 1 |
| GSM697400 |
P. aeruginosa intermittent infection isolate 5Mel replicate 2 |
|
| Relations |
| BioProject |
PRJNA139393 |
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