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Series GSE25199 Query DataSets for GSE25199
Status Public on Nov 15, 2011
Title HIF-1 and SKN-1 Coordinate the Transcriptional Response to Hydrogen Sulfide in C. elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Hydrogen sulfide (H2S) has dramatic physiological effects on animals that are associated with improved survival in changing conditions. C. elegans grown in H2S are long-lived and thermotolerant (1). To begin to identify mechanisms by which adaptation to H2S effects fundamental physiological functions, we have defined transcriptional changes associated with the response to H2S. Using microarray analysis we observe rapid changes in the abundance of specific mRNAs. The number and magnitude of transcriptional changes increased with the duration of H2S exposure. Functional annotation suggests that genes associated with protein homeostasis are upregulated upon prolonged exposure to H2S. Previous work has shown that the hypoxia-inducible transcription factor, HIF-1, is required for survival in H2S (2). We show that hif-1 is required for all early transcriptional changes in H2S. However, our data suggest that other factors may also regulate transcriptional effects of H2S exposure, and we demonstrate that SKN-1, the C. elegans homologue of NRF2, is also required for some H2S-dependent changes in transcription. Moreover, we have found that, like hif-1, skn-1 is essential to survive exposure to H2S. Our results support a model in which HIF-1 and SKN-1 coordinate a broad transcriptional response to H2S, with the end result to affect a global reorganization of protein homeostasis networks. We propose that these transcriptional responses contribute to the effects of adaptation to H2S on lifespan and thermotolerance.
 
Overall design A 14 chip study using total RNA isolated independently for each chip. 5 replicates of control untreated samples and 3 biological replicates for each treatement regimen were included
 
Contributor(s) Miller D, Budde M, Roth M
Citation(s) 21980473
Submission date Nov 08, 2010
Last update date Mar 22, 2012
Contact name Dana L Miller
E-mail(s) dlm16@uw.edu
Phone 206-685-5025
Organization name University of Washington
Department Biochemistry
Street address Box 357350, HSB J-587
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platforms (1)
GPL11171 NimbleGen Caenorhabditis elegans Gene expression 4x72K Array
Samples (3)
GSM619117 c elegans_h2s_1h
GSM619118 c elegans_h2s_12h
GSM619119 c elegans_h2s_48h
Relations
BioProject PRJNA134311

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25199_RAW.tar 16.9 Mb (http)(custom) TAR (of PAIR)
GSE25199_reference_pair_files.tar.gz 6.7 Mb (ftp)(http) TAR
Processed data included within Sample table

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