 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 10, 2010 |
| Title |
To determine the global gene expression response in murine LPS-induced macrophages treated with a bromodomain inhibitor. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Bone marrow-derived macrophages, Unstimulated DMSO Bone marrow-derived macrophages, Unstimulated + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 1h DMSO Bone marrow-derived macrophages, LPS 1h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 2h DMSO Bone marrow-derived macrophages, LPS 2h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 4h DMSO Bone marrow-derived macrophages, LPS 4h + I-BET (GSK525762A)
LPS (100 ng/mL) was purchased from Sigma. Bone Marrow-derived macrophages (BMDMs) were differentiated from C57BL/6 bone marrow using 5 ng/mL each of recombinant M-CSF and IL-3 (Peprotech) for 7 days as described (Jeffrey et al, Nature Immunology, 2006). 2 x10^6 BMDMs were treated with DMSO or 1 μM of I-BET for 30 minutes before the addition of LPS (100 ng/mL) for 1, 2 or 4h. Unstimulated control samples were incubated with I-BET only for 1 hour. 500 ng of total RNA from 3 independent samples per group was used to prepare biotin-labeled RNA using Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) and hybridized to Illumina MouseRef-8 v2.0 expression BeadChip kits. The chips were scanned using Illumina BeadArray Reader.
|
| |
|
| Overall design |
3 biological replicates and 4 timepoints
|
| |
|
| Contributor(s) |
Jeffrey K, Tarakhovsky A |
| Citation(s) |
21068722 |
| Submission date |
May 10, 2010 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Paul A Wilson |
| E-mail(s) |
paul.a.wilson@gsk.com
|
| Organization name |
GSK
|
| Department |
Target Sciences
|
| Lab |
Computational Biology
|
| Street address |
GlaxoSmithKline Medicine Research Centre
|
| City |
Stevenage |
| State/province |
Hertfordshire |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA127281 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21764_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE21764_non-normalized.txt.gz |
2.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...