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| Status |
Public on Dec 01, 2010 |
| Title |
Genetic analysis of the genome wide role of G-proteins in rice metabolism |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
The current study reports a genome-wide transcriptional profiling of the only known Gα mutants in rice (RGA1).
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| Overall design |
Total RNA was isolated from the leaves of 25 day old rice d1 (Gα) mutant (HO-541) and its corresponding wild type (Nipponbare) grown on B-5 agar media.Total RNAs from the rice Gα mutant and wild type were first converted into cDNAs, from which labeled cRNAs were generated with Cy3 and Cy5 dyes and hybridized with 44K 60-mer whole genome rice microarrays on glass slides.The slides were washed and scanned on an Agilent scanner (G2565B) at 100 % laser power and data extracted with Agilent Feature Extraction software (version 9.1). The experiment was repeated with a biological-cum-technical replicate, where total RNA samples from a fresh batch of rice (mutant and WT) plants were labeled with opposite dyes (dye-swap) and used for microarray analysis.
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| Contributor(s) |
Pathak R, Raghuram N |
| Citation(s) |
26858735 |
| Submission date |
Mar 17, 2010 |
| Last update date |
Feb 10, 2016 |
| Contact name |
Nandula Raghuram |
| E-mail(s) |
raghuram98@hotmail.com, ravipathak@aol.in, pathakster@gmail.com, navjyoti.chakraborty@gmail.com
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| Phone |
+91-11-25302308
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| Organization name |
Guru Gobind Singh Indraprastha University
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| Department |
School of Biotechnology
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| Lab |
Molecular Biology and Functional Genomics Lab
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| Street address |
Dwarka, Sector -16C
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| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110075 |
| Country |
India |
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| Platforms (1) |
| GPL10176 |
Oryza sativa 60-mer Oligonucleotide Platform |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA124361 |
