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| Status |
Public on Dec 11, 2009 |
| Title |
Profile of microRNA during adipogenesis regulating by microRNA and WNT signaling |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To investigate miRNAs that implicate the process of adipogenesis by interacting with canonical Wnt/β-catenin signaling pathway, we constructed two cell models at first, and then investigated the expression profile of microRNAs by using Microarray. Based on the data of high throughput microarray, we identified 18 miRNAs which might promote adipogenesis by repressing WNT signaling, including mir-210, mir-148a, mir-194, mir-322 etc. On the other hand, we also identified 29 miRNAs which might repress adipogenesis by activation of WNT signaling, including mir-344, mir-27, and mir-181. The target genes involved in WNT signaling pathway of these identified miRNAs were also predicted through online tools.
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| Overall design |
Two cell models of 3T3-L1, i.e. activation and suppression of WNT signaling including four samples, i.e. preadipocytes, mature adipocytes (MDI induction), Lithium treated preadipocytes, MDI induction of Lithium treated preadipocytes. Each sample was replicated in triplicate.
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| Contributor(s) |
Niu YN, Mo DL, Chen WQ, Wang QW, Xiao SQ, Xie Y, Li AN, Li J, Zhao X, He ZY |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 10, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Qin Limei |
| E-mail(s) |
wyqbj2004@163.com
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| Organization name |
SunYatsen University
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| Street address |
North Third road
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| City |
Guangzhou |
| ZIP/Postal code |
510006 |
| Country |
China |
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| Platforms (1) |
| GPL9756 |
Agilent-021828 Unrestricted Mouse miRNA Microarray (miRNA_105_Dec08) |
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| Samples (12)
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| GSM482978 |
mature adipocytes (MDI induction) B-1 |
| GSM482979 |
mature adipocytes (MDI induction) B-2 |
| GSM482980 |
mature adipocytes (MDI induction) B-3 |
| GSM482981 |
Lithium treated preadipocytes C-1 |
| GSM482982 |
Lithium treated preadipocytes C-2 |
| GSM482983 |
Lithium treated preadipocytes C-3 |
| GSM482984 |
MDI induction of Lithium treated preadipocytes D-1 |
| GSM482985 |
MDI induction of Lithium treated preadipocytes D-2 |
| GSM482986 |
MDI induction of Lithium treated preadipocytes D-3 |
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| Relations |
| BioProject |
PRJNA120359 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19421_RAW.tar |
9.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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