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| Status |
Public on Aug 18, 2009 |
| Title |
Expression data for UV time course in Saccharomyces cerevisiae wild-type and mutant strains |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Treatment of cells with DNA damaging agents leads to large-scale gene expression changes. Proper transcriptional regulation is important for cells to arrest, repair damage and adjust cellular processes such as metabolism in order to survive the damaging assault. Damage-induced transcription is a highly regulated response. This study establishes a novel role for two factors, Snf1 and Rad23, in regulation of the UV-induced transcriptional response.
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| Overall design |
Yeast strains were grown to log phase and irradiated with 100 J/m2 UV-C light. Unirradiated samples were also taken. Three different strains (WT, snf1Δ, mig3Δ) were analyzed in the absence of damage and at 15, 30 and 60 minutes following irradiation. Three other strains (rad23Δ, snf1Δ rad23Δ, rad4Δ) were analyzed in the absence of damage and at 30 and 60 minutes following irradiation for a total of 21 different strain/condition combinations. Experiments were performed in duplicate (two biological replicates for each condition). Samples were hybridized to Affymetrix Yeast 2.0 arrays. Undamaged WT samples were used as a control.
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| Contributor(s) |
Wade SL, Poorey K, Bekiranov S, Auble DT |
| Citation(s) |
19680226 |
| Submission date |
Jun 24, 2009 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Kunal Poorey |
| E-mail(s) |
kp9y@virginia.edu
|
| Phone |
434-982-3237
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| Fax |
434-924-5069
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| Organization name |
University of Virginia Health System
|
| Department |
Biomedical Sciences: Biochemistry, Molecular Biology & Genetics
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| Lab |
Auble Lab
|
| Street address |
1340 Jefferson Park Avenue
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (41)
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| Relations |
| BioProject |
PRJNA117509 |
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