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| Status |
Public on Jul 31, 2021 |
| Title |
Stem cell fate choices are regulated through antagonistic control of lysosomes by MYC and TFEB [RNA-Seq 5] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We show that lysosomes are antagonistically controlled by TFEB and MYC to balance catabolic and anabolic processes required for activating LT-HSC and guiding their lineage fate. TFEB-mediated induction of the endolysosomal pathway for membrane receptor degradation limits LT-HSC metabolic and mitogenic activation; this promotes quiescence and self-renewal and governs erythroid-myeloid commitment. By contrast, MYC engages biosynthetic processes while repressing lysosomal catabolism to drive LT-HSC activation. Collectively, our study identifies lysosomes as a central regulatory hub for proper and coordinated stem cell fate determination.
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| Overall design |
Short-term hematopoietic stem cells (ST-HSC) were sorted from human cord blood and cultured overnight before transduction with lentivirus to overexpress GP91 (CTRL) or TFEB. Three days later, BFP+ transduced cells were sorted for RNA extraction and sequencing.
Authors state that raw data will be uploaded to the EGA database.
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| Contributor(s) |
Garcia-Prat L |
| Citation missing |
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| Submission date |
Jul 07, 2020 |
| Last update date |
Jul 31, 2021 |
| Contact name |
Laura Garcia Prat |
| E-mail(s) |
laura.garciaprat@uhnresearch.ca
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| Organization name |
University Health Network
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| Department |
Princess Margaret Cancer Centre
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| Street address |
101 College street
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| City |
Toronto |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE153917 |
Stem cell fate choices are regulated through antagonistic control of lysosomes by MYC and TFEB |
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| Relations |
| BioProject |
PRJNA644531 |
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