 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 16, 2008 |
| Title |
Glioblastoma microvesicles transport RNA and proteins |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Glioblastoma tumour cells release microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by normal host cells, such as brain microvascular endothelial cells. By incorporating an mRNA for a reporter protein into these microvesicles, we demonstrate that messages delivered by microvesicles are translated by recipient cells. These microvesicles are also enriched in angiogenic proteins and stimulate tubule formation by endothelial cells. Tumour-derived microvesicles therefore serve as a means of delivering genetic information and proteins to recipient cells in the tumour environment. Glioblastoma microvesicles also stimulated proliferation of a human glioma cell line, indicating a self-promoting aspect. Messenger RNA mutant/variants and miRNAs characteristic of gliomas could be detected in serum microvesicles of glioblastoma patients. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma patients. Thus, tumour-derived microvesicles may provide diagnostic information and aid in therapeutic decisions for cancer patients through a blood test.
|
| |
|
| Overall design |
The glioblastoma cell and exosome RNA was analyzed on two dual color arrays.
|
| |
|
| Contributor(s) |
Skog J, Wurdinger T, van Rijn S, Meijer D, Gainche L, Sena-Esteves M, Curry WT, Carter BS, Krichevsky AM, Breakefield XO |
| Citation(s) |
19011622, 21285958 |
| Submission date |
Nov 04, 2008 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Johan Skog |
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Neurogenetics
|
| Lab |
XOB
|
| Street address |
149 13th street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA110001 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13470_RAW.tar |
35.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
