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Series GSE134387 Query DataSets for GSE134387
Status Public on Jan 31, 2020
Title Mediator condensates localize signaling factors to key cell identity genes
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The gene expression programs that define each cell’s identity are controlled by master transcription factors (TFs) that bind cell-type specific enhancers, as well as signaling factors, which bring extracellular stimuli to these enhancers. Recent studies have revealed that master TFs form phase-separated condensates with the Mediator coactivator at super-enhancers. Here we present evidence that signaling factors for the WNT, TGF-β and JAK/STAT pathways employ their intrinsically disordered regions (IDRs) to enter and concentrate in Mediator condensates at super-enhancers. We show that the WNT coactivator β-catenin interacts both with components of condensates and DNA binding factors to selectively occupy super-enhancer associated genes. We propose that the cell-type specificity of the response to signaling is mediated, in part, by the IDRs of the signaling factors, which cause these factors to partition into condensates established by the master TFs and Mediator at genes with prominent roles in cell identity.
 
Overall design beta_catenin, MED1, beta_catenin, armadillo, idrs ChIP-seq in mES cells grown in 2i.
 
Contributor(s) Zamudio AV
Citation(s) 31563432
Submission date Jul 17, 2019
Last update date Apr 10, 2020
Contact name Alicia Viridiana Zamudio
E-mail(s) zamudio.av@gmail.com
Phone 6199807656
Organization name Massachusetts Institute of Technology
Department Biology
Lab Young
Street address 455 Main St
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (5)
GSM3945588 20190523_8102: mES_GFP-bcatenin_GFP
GSM3945589 20190523_8101: mES_GFP-armadillo_GFP
GSM3945590 20190523_8100: mES_GFP-chimera_GFP
Relations
BioProject PRJNA555028
SRA SRP215004

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134387_RAW.tar 500.4 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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