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Status |
Public on Jan 31, 2020 |
Title |
Mediator condensates localize signaling factors to key cell identity genes |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The gene expression programs that define each cell’s identity are controlled by master transcription factors (TFs) that bind cell-type specific enhancers, as well as signaling factors, which bring extracellular stimuli to these enhancers. Recent studies have revealed that master TFs form phase-separated condensates with the Mediator coactivator at super-enhancers. Here we present evidence that signaling factors for the WNT, TGF-β and JAK/STAT pathways employ their intrinsically disordered regions (IDRs) to enter and concentrate in Mediator condensates at super-enhancers. We show that the WNT coactivator β-catenin interacts both with components of condensates and DNA binding factors to selectively occupy super-enhancer associated genes. We propose that the cell-type specificity of the response to signaling is mediated, in part, by the IDRs of the signaling factors, which cause these factors to partition into condensates established by the master TFs and Mediator at genes with prominent roles in cell identity.
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Overall design |
beta_catenin, MED1, beta_catenin, armadillo, idrs ChIP-seq in mES cells grown in 2i.
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Contributor(s) |
Zamudio AV |
Citation(s) |
31563432 |
Submission date |
Jul 17, 2019 |
Last update date |
Apr 10, 2020 |
Contact name |
Alicia Viridiana Zamudio |
E-mail(s) |
zamudio.av@gmail.com
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Phone |
6199807656
|
Organization name |
Massachusetts Institute of Technology
|
Department |
Biology
|
Lab |
Young
|
Street address |
455 Main St
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (5)
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Relations |
BioProject |
PRJNA555028 |
SRA |
SRP215004 |