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| Status |
Public on Jul 15, 2019 |
| Title |
CoBATCH for high-throughput single-cell epigenomic profiling [low input in situ ChIP] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
we develop a robut low input cell and high throughput single cell ChIP method used for both native and fixed in vitro cultured cells and intact tissue
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| |
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| Overall design |
we fused Protein A to the N-terminal of Tn5 to generated Protein A-Tn5 fusion enzyme, the ProteinA-Tn5-complex is enriched through specific antibodies to genomic regions and Tn5 generates indexed chromatin fragments ready for the library preparation and sequencing; ROC - receiver operating characteristic.
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| |
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| Contributor(s) |
Wang Q, Xiong H, Ai S, Yu X, Liu Y, Zhang J, He A |
| Citation(s) |
31471188 |
| Submission date |
Apr 04, 2019 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Haiqing Xiong |
| E-mail(s) |
haiqingxiong@pku.edu.cn
|
| Organization name |
Peking University
|
| Street address |
5 Yiheyuan Road, Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE129335 |
CoBATCH for high-throughput single-cell epigenomic profiling |
|
| Relations |
| BioProject |
PRJNA531012 |
| SRA |
SRP190918 |
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