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Status |
Public on Jun 04, 2019 |
Title |
Full-length mRNA sequencing reveals principles of poly(A) tail length control |
Organisms |
Caenorhabditis elegans; Homo sapiens; synthetic construct; synthetic RNA |
Experiment type |
Other
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Summary |
We report FLAM-seq, a cDNA library preparation method coupled to PacBio single-molecule sequencing for profiling full-length mRNAs including their poly(A) tail.
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Overall design |
FLAM-seq data for 2 replicates of HeLa S3 cells, iPSCs, cerebral organoids, C. elegans L4 and adult, and one replicate for synthetic RNA and cDNA standards.
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Contributor(s) |
Legnini I, Alles J, Karaiskos N, Ayoub S, Rajewsky N |
Citation(s) |
31384046, 36691625 |
Submission date |
Feb 12, 2019 |
Last update date |
Feb 07, 2023 |
Contact name |
Ivano Legnini |
Organization name |
MDC
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Department |
BIMSB
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Lab |
Systems Biology of Gene Regulatory Elements
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Street address |
Robert-Rössle-Straße 10, Haus 87
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City |
Berlin |
State/province |
Germany |
ZIP/Postal code |
13125 |
Country |
Germany |
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Platforms (4)
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Samples (12)
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Relations |
BioProject |
PRJNA522007 |
SRA |
SRP185743 |