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| Status |
Public on Jul 30, 2008 |
| Title |
Gene expression of CML CD34+ cells during Imatinib therapy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Imatinib has become the current standard therapy for patients with chronic myelogenous leukaemia (CML). For a better understanding of the Imatinib-related molecular effects in vivo, we assessed gene expression profiles of Philadelphia Chromosome positive (Ph+) CD34+ cells from peripheral blood of 6 patients with de novo CML in chronic phase. After 7 days of treatment with Imatinib the Ph+ CD34+ cells were reassessed to look for changes in the transcriptome. The expression level of 303 genes was significantly different comparing the transcriptome of the Ph+ CD34+ cells before and after 7 days of Imatinib therapy (183 down-regulated, 120 up-regulated, lower bound ≥1.2-fold). For a substantial number of genes governing cell cycle and DNA replication, the level of expression significantly decreased (CDC2, RRM2, PCNA, MCM4). On the other hand, therapy with Imatinib was associated with an increase of genes related to adhesive interactions, such as L-selectin or CD44. A group of 8 genes with differential expression levels were confirmed using a gene specific quantitative real-time PCR. Thus, during the first week of treatment, Imatinib is preferentially counteracting the bcr-abl induced effects related to a disturbed cell cycle and defective adhesion of leukemic Ph+ CD34+ cells.
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| Overall design |
In total 6 patients with new diagnosis CML (Chronic Myelogenous Leukemia) in chronic phase are inculded in the study. The gene expression profiles of the CD34+ hematopoietic stem and progenitor cells from the patients before first treatment with Glivec (Imatinib) are compared to the gene expression profiles of the CD34+ hematopoietic stem and progenitor cells of the same patients after 7 days of treatment with 400 mg Glivec / day.
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| Contributor(s) |
Bruennert DC, Czibere A, Bruns I, Kronenwett R, Gattermann N, Haas R, Neumann F |
| Citation(s) |
19052557 |
| Submission date |
Jul 23, 2008 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Daniela Bruennert |
| E-mail(s) |
Daniela.Bruennert@med.uni-duesseldorf.de
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| Phone |
0211/8114869
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| Organization name |
University Clinic Duesseldorf
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| Department |
Clinic for Hematology, Oncology and clin. Immunology
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| Lab |
Haematologisches Forschungslabor
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| Street address |
Universitaetsstr. 1
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| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA113723 |
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