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| Status |
Public on Jan 11, 2008 |
| Title |
Gene expression analysis of SMARTA in response to LCMV or Lm-gp61 infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Following infection with LCMV, CD4+ SMARTA TCR transgenic cells (specific for the gp61-80 epitope of the LCMV glycoprotein) rapidly expand, become effector cells, and go on to form a long-lived memory population. Following infection with a recombinant Listeria monocytogenes expressing the LCMV epitope gp61-80, SMARTA cells also expand but display defective effector differentiation and fail to form memory. In an attempt to understand the signals required for CD4 T cell memory differentiation, we compared gene expression by SMARTA cells at the peak of the primary response following either Lm-gp61 or LCMV infection.
Keywords: response to LCMV or Lm-gp61 infection
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| Overall design |
SMARTA cells were purified at day 7 post-infection with either LCMV or Lm-gp61. SMARTA cells were sorted on the basis of Thy1.1 expression using a FACSAria. Cells were sorted through the machine twice to enhance purity. Two biological replicates of each group are provided. Each replicate represents the results of SMARTA pooled from three animals.
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| Contributor(s) |
Williams MA, Bevan MJ |
| Citation(s) |
18356084 |
| Submission date |
Jan 07, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Matthew Williams |
| E-mail(s) |
matthew.williams@path.utah.edu
|
| Phone |
8015852487
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| Organization name |
University of Utah
|
| Department |
Pathology
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| Lab |
Williams
|
| Street address |
15 N. Medical Dr. E.
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA108663 |
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