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Platform GPL6871 Query DataSets for GPL6871
Status Public on Aug 26, 2008
Title University of Utah Schmidtea mediterranea cDNA array generation I
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Schmidtea mediterranea
Manufacturer Microarray Core Facility at the University of Utah
Manufacture protocol 3,435 cDNAs from two asexual cDNA libraries, a neoblast-enriched cell population (NB) and animal heads (H) (Sánchez Alvarado et al., Development. 129:5659-5665), were printed in triplicate on Corning UltraGAPS glass microscope slides using an Amersham BioSciences Lucidea Array Spotter. The PCR products were prepared for spotting by diluting the samples in a solution of 50% DMSO to an average final concentration of 100 ng/ul. Following the print session, the DNA targets were cross-linked to the slide surface at 250mJ using a Stratagene Stratalinker.
Support glass
Coating aminosilane
 
 
Contributor(s) Eisenhoffer GT, Kang H, Sánchez Alvarado A
Submission date May 17, 2008
Last update date Aug 26, 2008
Contact name Alejandro Sánchez Alvarado
E-mail(s) sanchez@neuro.utah.edu
URL http://planaria.neuro.utah.edu
Organization name University of Utah School of Medicine
Department Neurobiology and Anatomy
Street address 20N 1900E
City Salt Lake City
State/province UT
ZIP/Postal code 84132-3401
Country USA
 
Samples (11) GSM289255, GSM289256, GSM289257, GSM289258, GSM289259, GSM289260 
Series (1)
GSE11503 Expression Profiles of Planarain Stem Cells and their Immediate Division Progeny

Data table header descriptions
ID
EST ID The letters H and NB under EST ID refer to Head- and Neoblast-enriched cDNA libraries, while the alphanumerical code refers to the 96-well plate coordinates.
Swiss Prot ID and Organism Primary Swiss Prot Accession Number, Swiss Prot Entry Name, and Organism
Gene Description Gene Description refers to homology with proteins encoded in the genomes of other organisms
Evalue Expect value from BLASTX program
GB_ACC GenBank Accession number

Data table
ID EST ID Swiss Prot ID and Organism Gene Description Evalue GB_ACC
AY068380 H.1.10e sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock protein 90 kDa beta member 1) (Heat shock 108 kDa protein) (HSP108) (HSP 108) (Transferrin-binding protein) 2.6E-28 AY068380
AY066533 H.1.10g No Match No Match No Match AY066533
AY068381 H.1.11b sp|P20646|PPAP_RAT Prostatic acid phosphatase precursor 9.8E-11 AY068381
AY066534 H.1.11e No Match No Match No Match AY066534
FG311481 H.1.12b No Match No Match No Match FG311481
FG311482 H.1.12c No Match No Match No Match FG311482
AY068366 H.1.12e sp|Q3U962|CO5A2_MOUSE Collagen alpha-2(V) chain precursor 0.000011 AY068366
AY066535 H.1.12g No Match No Match No Match AY066535
FG311483 H.1.1e No Match No Match No Match FG311483
AY068383 H.1.1g sp|Q8IWB7|WDFY1_HUMAN WD repeat and FYVE domain-containing protein 1 (WD40- and FYVE domain-containing protein 1) (Phosphoinositide-binding protein 1) (FENS-1) (Zinc finger FYVE domain-containing protein 17) 1.9E-10 AY068383
AY068384 H.1.3a sp|P24733|MYS_AEQIR Myosin heavy chain, striated muscle 8.2E-20 AY068384
AY067799 H.1.3b sp|Q6ZRI0.2|OTOGL_HUMAN Otogelin precursor 0.00006 AY067799
FG311569 H.1.3e sp|P18160.3|KYK1_DICDI Non-receptor tyrosine kinase spore lysis A (Tyrosine-protein kinase 1) 4.3E-13 FG311569
FG311484 H.1.4b sp|P34127.2|MYBA_DICDI Myb-like protein A 9.7E-22 FG311484
AY067806 H.1.4g sp|P50431|GLYC_MOUSE Serine hydroxymethyltransferase, cytosolic (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT) 4E-21 AY067806
AY068723 H.1.4h No Match No Match No Match AY068723
FG311485 H.1.5e No Match No Match No Match FG311485
AY967471 H.1.5f sp|Q3SYV4|CAP1_BOVIN Adenylyl cyclase-associated protein 1 (CAP 1) 2E-50 AY967471
AY066306 H.1.6f No Match No Match No Match AY066306
AY967472 H.1.6h sp|P68371|TBB2C_HUMAN Tubulin beta-2C chain (Tubulin beta-2 chain) >sp|P68372|TBB2C_MOUSE Tubulin beta-2C chain >sp|Q3MHM5|TBB2C_BOVIN Tubulin beta-2C chain 1.3E-103 AY967472

Total number of rows: 3435

Table truncated, full table size 331 Kbytes.




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