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  This describes how to format marker data to submit it to UniSTS.

The file name should be [mapname]_marker_data.
If the map name contains space, replace the space with _ (underscore) in [mapname]_marker_desc.
For example, if your map name is "My Map", the file name should be My_Map_marker_data.

Provide these data as tab-delimited fields and save it as text file.

***** if there is no value for a field, leave it empty rather than writing na - ? etc. ****

1. marker name: Marker name as appears in column 5 of [mapname]_map_data.txt file.

2. marker type: STS, MICROSATELLITE, GENE, RFLP, RAPD, or AFLP.
If non of the above, contact UniSTS submission.

3. forward primer: Use IUPAC nucleotide symbols.

4. reverse primer: Use IUPAC nucleotide symbols. Combination of forward primer and reverse primer should have only one line in each map.

5. pcr product size: If range, specify with dash, e.g., 120-140.

6. GenBank accession: Put accession here if the marker is submitted to STS division of GenBank, or if marker is derived from a GenBank accession. If provided value is not an genbank accession, it is ignored.

7. marker xref id: Unique identifier of the marker in other database, if applicable.
It should contain source database and id, e.g., MGD:1234 or RGD:1234 or GDB:1234 or MARC:1234. This is to provide links to other datasources. Please note that there is no checks on the value of xrefs.

8. alias: Marker alias other than name in column 1.
e.g., DXS7530;G04642;WI-4463;MR7417;GDB:589110

9. taxonomy id: List it if it is different from the organism of the map data.

10. pubmed id: Pubmed identifier, e.g., 12640126;11390421

 

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