USDA-ARS Bovine Full-Length Insert cDNA Project

OVERVIEW

The Genetics and Breeding Research Unit (GBRU) at the United States Meat Animal Research Center (USMARC) and the Bovine Functional Genomics Laboratory (BFGL) at the Beltsville Area Research Center (BARC)* collaborated to generate 265,776 5' EST sequences from eight bovine pooled-tissue libraries, three of which were created using full-length enrichment strategies. The objective of this project was to identify a set of clones predicted to contain the full CDS of the cognate gene, and sequence over 1000 bovine full-length insert cDNAs (bFLICs) representing unique bovine genes. To date, 1304 bFLICs have been submitted to Genbank, representing 1045 unique bovine genes. Most clones (from libraries designated 1BOV through 5BOV) are distributed by BACPAC Resources - Children's Hospital Oakland Research Institute, while clones from the full-length enriched libraries (8BOV through 10BOV) are distributed by Tad Sonstegard at BARC (request from tads@anri.barc.usda.gov).

CONTACT

Gregory Harhay, USDA-ARS-USMARC

REFERENCES

Smith TPL, et al. Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle. Genome Res. 2001 Apr;11(4):626-30.

Sonstegard TS, et al. Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index. Mamm Genome. 2002 Jul;13(7):373-9.

Harhay GP, et al. Characterization of 954 bovine full-CDS cDNA sequences. BMC Genomics. 2005 Nov 23;6:166..

LINKS

Genetics and Breeding Research Unit (GBRU), MARC

Bovine Functional Genomics Laboratory (BFGL), BARC

Clone ordering information for BACPAC Resources

cDNA Sequence Search

NCBI Bovine Genome Resources

NCBI Genome Project Overview