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    dctA C4 dicarboxylate/orotate:H(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948039, updated on 4-Apr-2024

    Summary

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    Gene symbol
    dctA
    Gene description
    C4 dicarboxylate/orotate:H(+) symporter
    Primary source
    ASAP:ABE-0011527
    Locus tag
    b3528
    See related
    ECOCYC:EG20044
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3513
    Summary
    DctA is required for dicarboxylate transport . [More information is available at EcoCyc: EG20044].
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    Genomic context

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    Sequence:
    NC_000913.3 (3682161..3683447, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-dehydro-3-deoxygluconokinase Neighboring gene peptidase M16 family protein YhjJ Neighboring gene c-di-GMP phosphodiesterase PdeK Neighboring gene cellulose synthase outer membrane channel Neighboring gene endo-1,4-D-glucanase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Genetic control of the uptake of C(4)-dicarboxylic acids by Escherichia coli. Kay WW, et al. FEBS Lett, 1969 Apr. PMID 11946977
    2. CRP interacts with promoter-bound sigma54 RNA polymerase and blocks transcriptional activation of the dctA promoter. Wang YP, et al. EMBO J, 1998 Feb 2. PMID 9451003, Free PMC Article
    3. Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA. Baker KE, et al. J Bacteriol, 1996 Dec. PMID 8955389, Free PMC Article
    4. Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations. Rayman MK, et al. J Biol Chem, 1972 Oct 10. PMID 4568614
    5. Genetic analysis of mutants of Escherichia coli defective in dicarboxylate transport. Lo TC, et al. Mol Gen Genet, 1975 Oct 22. PMID 1107801

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. In this work, it is shown that DctA is required for aerobic growth with L-aspartate as a carbon source.
      Title: DcuA of aerobically grown Escherichia coli serves as a nitrogen shuttle (L-aspartate/fumarate) for nitrogen uptake.
    2. The relationship of absolute contents of DcuS, DcuB, and DctA to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo, which was not accessible by other approaches, leading to a revision of previous models.
      Title: Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR.
    3. DcuS is required for location of DctA and DcuR at the poles and formation of tripartite DctA/DcuS/DcuR sensor/regulator complexes.
      Title: Polar localization of a tripartite complex of the two-component system DcuS/DcuR and the transporter DctA in Escherichia coli depends on the sensor kinase DcuS.
    4. C4-dicarboxylate sensing by DctA/DcuS resides in DcuS, and that DctA serves as an activity switch.
      Title: Differentiation of DctA and DcuS function in the DctA/DcuS sensor complex of Escherichia coli: function of DctA as an activity switch and of DcuS as the C4-dicarboxylate sensor.
    5. Reorganizing the DcuS homodimer and its interaction with DctA activate the kinase.
      Title: The cytoplasmic PASC domain of the sensor kinase DcuS of Escherichia coli: role in signal transduction, dimer formation, and DctA interaction.
    6. It is concluded that DctA forms a functional unit or sensor complex with DcuS through specific interaction sites.
      Title: Interaction of the Escherichia coli transporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units.
    Submit: New GeneRIF Correction

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables L-aspartate transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables fumarate transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables fumarate transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables malate:proton symporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables malate:proton symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables succinate transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables succinate transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in L-aspartate transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within L-aspartate transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in carboxylic acid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in dicarboxylic acid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within fumarate transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within fumarate transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in malate transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in succinate transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    C4 dicarboxylate/orotate:H(+) symporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3682161..3683447 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417985.1 C4 dicarboxylate/orotate:H(+) symporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417985.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MKU7, A0A5B9ACJ5
      Conserved Domains (1) summary
      PRK01663
      Location:1 → 427
      PRK01663; C4-dicarboxylate transporter DctA; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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    Other Literature Sources
    Molecular Biology Databases