Usp5 ubiquitin specific peptidase 5 (isopeptidase T) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Usp5provided by MGI
Official Full Name: ubiquitin specific peptidase 5 (isopeptidase T)provided by MGI
Primary source: MGI:MGI:1347343
See related: Ensembl:ENSMUSG00000038429 AllianceGenome:MGI:1347343
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ISOT; Ucht; ISOT-1
Summary: Predicted to enable cysteine-type endopeptidase activity; thiol-dependent deubiquitinase; and ubiquitin binding activity. Predicted to be involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process and protein K48-linked deubiquitination. Predicted to act upstream of or within proteolysis. Predicted to be active in cytosol and nucleus. Orthologous to human USP5 (ubiquitin specific peptidase 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 37.6), whole brain E14.5 (RPKM 37.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 F2; 6 59.17 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (124791982..124806404, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (124815019..124829484, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.
Title: ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.
USP5 knockdown alleviates lung cancer progression via activating PARP1-mediated mTOR signaling pathway.
Title: USP5 knockdown alleviates lung cancer progression via activating PARP1-mediated mTOR signaling pathway.
USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity.
Title: USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicity.
Macrophage-derived extracellular vesicles regulates USP5-mediated HDAC2/NRF2 axis to ameliorate inflammatory pain.
Title: Macrophage-derived extracellular vesicles regulates USP5-mediated HDAC2/NRF2 axis to ameliorate inflammatory pain.
dysregulation of USP5 SUMOylation after peripheral nerve injury may contribute to the well described alteration in Cav3.2 channel activity
Title: SUMOylation regulates USP5-Cav3.2 calcium channel interactions.
USP5 mediated dysregulation of Cav3.2 channel activity does not exhibit sex differences, and potential therapeutics targeting this interaction should be effective in both male and female subjects.
Title: Disrupting USP5/Cav3.2 interactions protects female mice from mechanical hypersensitivity during peripheral inflammation.
identify interleukin-1 beta as an upstream trigger for the upregulation of interactions between USP5 and Cav3.2 channels in the pain pathway
Title: Identification of interleukin-1 beta as a key mediator in the upregulation of Cav3.2-USP5 interactions in the pain pathway.
Sensitization was relieved by pharmacological block of TRPV1 afferents, but not of myelinated neurons. In spinal cord slice recordings, we could optogenetically trigger an activity-dependent potentiation of presynaptic neurotransmission in the spinal dorsal horn that relied on Cav3.2 channel activity. This neuronal-activity-induced USP5 upregulation may underlie a protective, transient sensitization of the pain pathway.
Title: TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity.
Suramin and Cav3.2/USP5 Tat-disruptor peptides were also tested in models of diabetic neuropathy and visceral pain, and provided remarkable protection
Title: Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.
Knockdown and overexpression studies demonstrated that Usp5 regulates p53 (and p73) levels and alters cell growth and cell cycle distribution associated with p21 induction.
Title: Usp5 links suppression of p53 and FAS levels in melanoma to the BRAF pathway.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Transgenic (1)
Gene trapped (1)
Targeted (1)
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MARC_3767-3768:991937568:3 (e-PCR)
- UniSTS:231055

RH124604 (e-PCR)
- UniSTS:161426

MARC_7181-7182:996687603:1 (e-PCR)
- UniSTS:269724

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cysteine-type deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in negative regulation of ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein deubiquitination	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation protein catabolic process at presynapse	EXP Inferred from Experiment more info	PubMed
involved_in regulation protein catabolic process at presynapse	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation protein catabolic process at presynapse	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation protein catabolic process at presynapse	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ubiquitin carboxyl-terminal hydrolase 5

Names: deubiquitinating enzyme 5; isopeptidase T; ubiquitin carboxy terminal hydrolase T; ubiquitin specific protease 5; ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific-processing protease 5

NP_001313523.1

EC 3.4.19.12

NP_001397416.1

EC 3.4.19.12

NP_001397417.1

EC 3.4.19.12

NP_001397418.1

EC 3.4.19.12

NP_001397419.1

EC 3.4.19.12

NP_001397420.1

EC 3.4.19.12

NP_001397421.1

EC 3.4.19.12

NP_001397422.1

EC 3.4.19.12

NP_038728.1

EC 3.4.19.12

XP_006505979.1

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001326594.2 → NP_001313523.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2), is shorter, but has the same N- and C-termini as isoform 1.

Source sequence(s)

AC142254

Consensus CDS

CCDS85157.1

UniProtKB/TrEMBL

Q3U4W8

Related

ENSMUSP00000114000.2, ENSMUST00000122110.8

Conserved Domains (5) summary

COG5207
Location:18 → 832

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

cd02658
Location:327 → 831

Peptidase_C19B; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cd14383
Location:632 → 679

UBA1_UBP5; UBA1 domain found in ubiquitin carboxyl-terminal hydrolase 5 (UBP5)

cd14386
Location:700 → 742

UBA2_UBP5; UBA2 domain found in ubiquitin carboxyl-terminal hydrolase 5 (UBP5)

pfam02148
Location:199 → 272

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
NM_001410487.1 → NP_001397416.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 3

Status: VALIDATED

Source sequence(s)

AC142254
NM_001410488.1 → NP_001397417.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 4

Status: VALIDATED

Source sequence(s)

AC142254
NM_001410489.1 → NP_001397418.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 5

Status: VALIDATED

Source sequence(s)

AC142254
NM_001410490.1 → NP_001397419.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 5

Status: VALIDATED

Source sequence(s)

AC142254
NM_001410491.1 → NP_001397420.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 6

Status: VALIDATED

Source sequence(s)

AC142254
NM_001410492.1 → NP_001397421.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 6

Status: VALIDATED

Source sequence(s)

AC142254
NM_001410493.1 → NP_001397422.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 6

Status: VALIDATED

Source sequence(s)

AC142254
NM_013700.3 → NP_038728.1 ubiquitin carboxyl-terminal hydrolase 5 isoform 1

See identical proteins and their annotated locations for NP_038728.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC142254

Consensus CDS

CCDS20531.1

UniProtKB/Swiss-Prot

P56399

Related

ENSMUSP00000041299.4, ENSMUST00000047510.10

Conserved Domains (5) summary

COG5207
Location:18 → 855

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

cd02658
Location:327 → 854

Peptidase_C19B; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cd14383
Location:655 → 702

UBA1_UBP5; UBA1 domain found in ubiquitin carboxyl-terminal hydrolase 5 (UBP5)

cd14386
Location:723 → 765

UBA2_UBP5; UBA2 domain found in ubiquitin carboxyl-terminal hydrolase 5 (UBP5)

pfam02148
Location:199 → 272

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

124791982..124806404 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006505916.5 → XP_006505979.1 ubiquitin carboxyl-terminal hydrolase 5 isoform X1

Conserved Domains (3) summary

COG5207
Location:71 → 809

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

cd02658
Location:281 → 808

Peptidase_C19B; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam17807
Location:1 → 33

zf-UBP_var; Variant UBP zinc finger