Syt1 synaptotagmin I [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Syt1provided by MGI
Official Full Name: synaptotagmin Iprovided by MGI
Primary source: MGI:99667
See related: Ensembl:ENSMUSG00000035864; Vega:OTTMUSG00000016266
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SytI; AW124717; G630098F17Rik

Genomic context

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Location :: 10 D1; 10 56.52 cM

Sequence :: Chromosome: 10; NC_000076.6 (108497650..109010975, complement)

See Syt1 in Epigenomics, MapViewer

Chromosome 10 - NC_000076.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesicles.
Title: Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesicles.
APP interacts with the calcium sensor synaptotagmin-1 of synaptic vesicles and might thus play a role in the regulation of synaptic vesicle exocytosis.
Title: Interactome of the amyloid precursor protein APP in brain reveals a protein network involved in synaptic vesicle turnover and a close association with Synaptotagmin-1.
SNAREpin assembly by Munc18-1 requires previous vesicle docking by synaptotagmin 1.
Title: SNAREpin assembly by Munc18-1 requires previous vesicle docking by synaptotagmin 1.
Data suggest that Syt1 may act as a MTOC-associated protein to play important roles in mouse oocyte spindle organization/stability, and that it is indispensable for the metaphase-anaphase transition to promote mouse oocyte meiotic maturation.
Title: Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes.
The dynamics of vesicle fission during clathrin-mediated endocytosis is Ca(2)+ dependent but becomes Ca(2)+ independent in synaptotagmin 1 knock-out cells.
Title: Synaptotagmin 1 is necessary for the Ca2+ dependence of clathrin-mediated endocytosis.
This study demonistrated that syt1 functions as a dual Ca(2+) sensor for both endo- and exocytosis, potentially coupling these two components of the vesicle cycle.
Title: Uncoupling the roles of synaptotagmin I during endo- and exocytosis of synaptic vesicles.
This system recapitulates some of the effects of syt mutations that alter synaptic transmission in neurons. It demonstrate that the cytoplasmic domain of syt exhibited mixed agonist/antagonist activity during regulated membrane fusion in cells.
Title: Reconstituted synaptotagmin I mediates vesicle docking, priming, and fusion.
this study demonistrated that Alcohol induces synaptotagmin 1 expression in neurons via activation of heat shock factor 1
Title: Alcohol induces synaptotagmin 1 expression in neurons via activation of heat shock factor 1.
A test of Syt1 exocytosis in otoferlin-deficient hippocampal neurons and Syt1-deficient inner hair cells finds no deficits in vesicle fusion.
Title: Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
new-synthesized synaptotagmin1 following synaptic plasticity may contribute to long-lasting synaptic plasticity via positive, feedfoward mechanisms
Title: Synaptotagmin1 synthesis induced by synaptic plasticity in mouse hippocampus through activation of nicotinic acetylcholine receptors.

Submit: New GeneRIF Correction See all (46)

Alleles

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Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (1)
Targeted (knock-out) (1) 1 citation
Targeted (knock-in) (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description
BioGRID:203611	BioGRID:200268	Htt		BioGRID	PubMed	Affinity Capture-MS
BioGRID:203611	BioGRID:203609	Syp		BioGRID	PubMed	Co-fractionation

Pathways from BioSystems

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Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
GABA synthesis, release, reuptake and degradation, organism-specific biosystem (from REACTOME)
GABA synthesis, release, reuptake and degradation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Glutamate Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Glutamate Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Neuronal System, organism-specific biosystem (from REACTOME)
Neuronal System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Norepinephrine Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Norepinephrine Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Synaptic vesicle cycle, organism-specific biosystem (from KEGG)
Synaptic vesicle cycle, organism-specific biosystemCommunication between neurons is mediated by the release of neurotransmitter from synaptic vesicles (SVs). At the nerve terminal, SVs cycle through repetitive episodes of exocytosis and endocytosis. ...
Synaptic vesicle cycle, conserved biosystem (from KEGG)
Synaptic vesicle cycle, conserved biosystemCommunication between neurons is mediated by the release of neurotransmitter from synaptic vesicles (SVs). At the nerve terminal, SVs cycle through repetitive episodes of exocytosis and endocytosis. ...
Transmission across Chemical Synapses, organism-specific biosystem (from REACTOME)
Transmission across Chemical Synapses, organism-specific biosystemcomputationally inferred pathway (not manually curated)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystem (from WikiPathways)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystemXPodNet is a larger version of PodNet. Interactions from STRING database have been added to PodNet to build XPodNet. Hence, not all interactions found in XPodNet do necessarily have to be curated.

General gene information

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Markers

Syt1 (e-PCR), detects polymorphism
- UniSTS:546590

NoName (e-PCR)
- UniSTS:492877

RH94645 (e-PCR)
- UniSTS:119892

RH78549 (e-PCR)
- UniSTS:63327

Syt1 (e-PCR), detects polymorphism
- UniSTS:507257

AW124717 (e-PCR)
- UniSTS:180117

Syt1 (e-PCR), detects polymorphism
- UniSTS:498326

D37792 (e-PCR)
- UniSTS:124827

RH140034 (e-PCR)
- UniSTS:222116

Homology

Homologs of the Syt1 gene: The Syt1 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, fruit fly, mosquito, and C.elegans.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
SNARE binding	ISO Inferred from Sequence Orthology more info
calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
calcium ion binding	ISO Inferred from Sequence Orthology more info
calcium-dependent phospholipid binding	ISO Inferred from Sequence Orthology more info
calcium-dependent phospholipid binding	TAS Traceable Author Statement more info	PubMed
calcium-dependent protein binding	IPI Inferred from Physical Interaction more info	PubMed
calcium-dependent protein binding	ISO Inferred from Sequence Orthology more info
calmodulin binding	ISO Inferred from Sequence Orthology more info
clathrin binding	ISO Inferred from Sequence Orthology more info
identical protein binding	ISO Inferred from Sequence Orthology more info
low-density lipoprotein particle receptor binding	ISO Inferred from Sequence Orthology more info	PubMed
metal ion binding	IEA Inferred from Electronic Annotation more info
phosphatidylinositol-4,5-bisphosphate binding	ISO Inferred from Sequence Orthology more info
phosphatidylserine binding	ISO Inferred from Sequence Orthology more info
phospholipid binding	ISO Inferred from Sequence Orthology more info
protein C-terminus binding	ISO Inferred from Sequence Orthology more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
syntaxin binding	ISO Inferred from Sequence Orthology more info
syntaxin-1 binding	ISO Inferred from Sequence Orthology more info
transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
detection of calcium ion	ISO Inferred from Sequence Orthology more info
neurotransmitter secretion	IDA Inferred from Direct Assay more info	PubMed
positive regulation of calcium ion-dependent exocytosis	ISO Inferred from Sequence Orthology more info
positive regulation of vesicle fusion	ISO Inferred from Sequence Orthology more info
regulation of calcium ion-dependent exocytosis	ISO Inferred from Sequence Orthology more info
response to calcium ion	ISO Inferred from Sequence Orthology more info
synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info
transport	IEA Inferred from Electronic Annotation more info
vesicle docking	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
cell junction	IEA Inferred from Electronic Annotation more info
cytoplasm	ISO Inferred from Sequence Orthology more info
cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
dense core granule	ISO Inferred from Sequence Orthology more info
excitatory synapse	ISO Inferred from Sequence Orthology more info
integral to membrane	IEA Inferred from Electronic Annotation more info
intracellular organelle	ISO Inferred from Sequence Orthology more info
membrane	IEA Inferred from Electronic Annotation more info
neuron projection	IDA Inferred from Direct Assay more info	PubMed
plasma membrane	ISO Inferred from Sequence Orthology more info
plasma membrane	TAS Traceable Author Statement more info	PubMed
presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
secretory granule	IDA Inferred from Direct Assay more info	PubMed
secretory granule	ISO Inferred from Sequence Orthology more info
synapse	IEA Inferred from Electronic Annotation more info
synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
synaptic vesicle	ISO Inferred from Sequence Orthology more info
synaptic vesicle	TAS Traceable Author Statement more info	PubMed
synaptic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: synaptotagmin-1

Names: synaptotagmin-1; p65; synaptotagmin 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252341.1 → NP_001239270.1 synaptotagmin-1 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and it encodes the longer protein (isoform 1). Variants 1 and 2 encode the same protein.

Source sequence(s)

AI842617, AK078790, BC042519, BY268027, CD774438

Consensus CDS

CCDS24163.1

UniProtKB/TrEMBL

H6RXZ1

UniProtKB/Swiss-Prot

P46096

Related

ENSMUSP00000100912, OTTMUSP00000017428, ENSMUST00000105276, OTTMUST00000039005

Conserved Domains (2) summary

cd08385
Location:142 – 264
Blast Score: 664

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:273 – 408
Blast Score: 671

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1
NM_001252342.1 → NP_001239271.1 synaptotagmin-1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR and uses a difference splice site in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

Source sequence(s)

AI842617, AK018163, AK133578, BC042519, BY268027, CD774438

UniProtKB/Swiss-Prot

P46096

Conserved Domains (2) summary

cd08385
Location:139 – 261
Blast Score: 664

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:270 – 405
Blast Score: 671

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1
NM_009306.3 → NP_033332.1 synaptotagmin-1 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).

Source sequence(s)

AI842617, BC042519, BY238701, BY268027, CD774438

Consensus CDS

CCDS24163.1

UniProtKB/TrEMBL

H6RXZ1

UniProtKB/Swiss-Prot

P46096

Related

ENSMUSP00000063293, OTTMUSP00000021532, ENSMUST00000064054, OTTMUST00000047565

Conserved Domains (2) summary

cd08385
Location:142 – 264
Blast Score: 664

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:273 – 408
Blast Score: 671

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000076.6 Reference GRCm38.p1 C57BL/6J

Range

108497650..109010975, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000032.1 Alternate Mm_Celera

Range

110438514..110956520, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01088709.1 (6479..18060)	None
genomic	AAHY01088710.1	None
genomic	AAHY01088711.1	None
genomic	AAHY01088712.1	None
genomic	AAHY01088713.1	None
genomic	AAHY01088714.1	None
genomic	AAHY01088715.1	None
genomic	AAHY01088716.1	None
genomic	AAHY01088717.1	None
genomic	AAHY01088718.1	None
genomic	AAHY01088719.1	None
genomic	AAHY01088720.1	None
genomic	AAHY01088721.1	None
genomic	AAHY01088722.1	None
genomic	AAHY01088723.1	None
genomic	AAHY01088724.1	None
genomic	AAHY01088725.1	None
genomic	AAHY01088726.1	None
genomic	AAHY01088727.1	None
genomic	AAHY01088728.1	None
genomic	AAHY01088729.1	None
genomic	AAHY01088730.1	None
genomic	AAHY01088731.1	None
genomic	AAHY01088732.1	None
genomic	AAHY01088733.1	None
genomic	AAHY01088734.1 (4664..9945)	None
genomic	AC117199.3 (116521..210514)	None
genomic	AC122472.5 (100637..131094)	None
genomic	AC124436.5 (146900..174497)	None
genomic	AC141899.4 (110575..210306)	None
genomic	AC162908.4 (53003..170988)	None
genomic	AC163645.5 (70171..212773)	None
genomic	CH466539.1	EDL21707.1	mixed
		EDL21708.1	mixed
genomic	CS667543.1	CAP07395.1
mRNA	AI842617.1	None	C57BL/6J
mRNA	AK013798.1	None	C57BL/6J
mRNA	AK018163.1	None	C57BL/6J
mRNA	AK019304.1	None	C57BL/6J
mRNA	AK032444.1	None	C57BL/6J
mRNA	AK048973.1	None	C57BL/6J
mRNA	AK078790.1	BAC37395.1	C57BL/6J
mRNA	AK090394.1	None	C57BL/6J
mRNA	AK133578.1	None	C57BL/6J
mRNA	AK164153.1	BAE37652.1	C57BL/6J
mRNA	AK165749.1	None	C57BL/6J
mRNA	BC028277.1	None
mRNA	BC042519.1	AAH42519.1
mRNA	BC048187.1	None	C57BL/6
mRNA	BC058423.1	None	C57BL/6
mRNA	BY238701.1	None	C57BL/6J
mRNA	BY268027.1	None	C57BL/6J
mRNA	CD774438.1	None	C57BL/6J
mRNA	D37792.1	BAA07040.1
mRNA	FR827897.1	CBZ98840.1	Ola129xC57BL6J