Gls glutaminase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Glsprovided by MGI
Official Full Name: glutaminaseprovided by MGI
Primary source: MGI:MGI:95752
See related: Ensembl:ENSMUSG00000026103 AllianceGenome:MGI:95752
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 6330442B14; B230365M23Rik
Summary: Enables glutaminase activity and identical protein binding activity. Involved in glutamate biosynthetic process; glutamine catabolic process; and protein homotetramerization. Acts upstream of or within chemical synaptic transmission; regulation of respiratory gaseous exchange by nervous system process; and suckling behavior. Predicted to be located in mitochondrion. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 71. Orthologous to human GLS (glutaminase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E18 (RPKM 20.0), cortex adult (RPKM 19.9) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 C1.1; 1 26.86 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (52202608..52274168, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (52163449..52235009, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren's Syndrome.
Title: Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren's Syndrome.
Microglial glutaminase 1 deficiency mitigates neuroinflammation associated depression.
Title: Microglial glutaminase 1 deficiency mitigates neuroinflammation associated depression.
Targeting Hepatic Glutaminase 1 Ameliorates Non-alcoholic Steatohepatitis by Restoring Very-Low-Density Lipoprotein Triglyceride Assembly.
Title: Targeting Hepatic Glutaminase 1 Ameliorates Non-alcoholic Steatohepatitis by Restoring Very-Low-Density Lipoprotein Triglyceride Assembly.
SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells.
Title: SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells.
Nrf2 Activation Sensitizes K-Ras Mutant Pancreatic Cancer Cells to Glutaminase Inhibition.
Title: Nrf2 Activation Sensitizes K-Ras Mutant Pancreatic Cancer Cells to Glutaminase Inhibition.
The activation loop and substrate-binding cleft of glutaminase C are allosterically coupled.
Title: The activation loop and substrate-binding cleft of glutaminase C are allosterically coupled.
STAT1 upregulates glutaminase and modulates amino acids and glutathione metabolism.
Title: STAT1 upregulates glutaminase and modulates amino acids and glutathione metabolism.
Spontaneous colitis in IL-10-deficient mice was ameliorated via inhibiting glutaminase1.
Title: Spontaneous colitis in IL-10-deficient mice was ameliorated via inhibiting glutaminase1.
Using the conditional-knockout mice, we demonstrated that both in vitro Th17 differentiation (P < 0.05) and the development of experimental autoimmune encephalitis were dependent on Gls1.
Title: Glutaminase 1 Inhibition Reduces Glycolysis and Ameliorates Lupus-like Disease in MRL/lpr Mice and Experimental Autoimmune Encephalomyelitis.
results show that GLS1 mutant mice display moderate impairments of hippocampal glutamatergic neurotransmission and moderate changes in adaptive behaviors that have been shown to participate to the development of depressive-like state.
Title: GLS1 Mutant Mice Display Moderate Alterations of Hippocampal Glutamatergic Neurotransmission Associated with Specific Adaptive Behavioral Changes.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (7)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Gls (e-PCR), detects polymorphism
- UniSTS:506938

AI314027 (e-PCR)
- UniSTS:158792

Gls (e-PCR), detects polymorphism
- UniSTS:256613

Gls (e-PCR), detects polymorphism
- UniSTS:143224

D1Ncl2 (e-PCR), detects polymorphism
- UniSTS:142261

RH137240 (e-PCR)
- UniSTS:219328

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables glutaminase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutaminase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutaminase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glutaminase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutamate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glutamate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in glutamine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within glutamine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutamine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in glutamine metabolic process	IEA Inferred from Electronic Annotation more info
involved_in intracellular glutamate homeostasis	ISO Inferred from Sequence Orthology more info
involved_in protein homotetramerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein homotetramerization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of respiratory gaseous exchange by nervous system process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within suckling behavior	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutaminase kidney isoform, mitochondrial

NP_001074550.1

EC 3.5.1.2

NP_001106854.1

EC 3.5.1.2

XP_006495764.1

EC 3.5.1.2

XP_006495765.1

EC 3.5.1.2

XP_006495767.1

EC 3.5.1.2

XP_017171725.1

EC 3.5.1.2

XP_036016518.1

EC 3.5.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081081.2 → NP_001074550.1 glutaminase kidney isoform, mitochondrial isoform 1

See identical proteins and their annotated locations for NP_001074550.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC123752

Consensus CDS

CCDS35561.1

UniProtKB/Swiss-Prot

D3Z7P3, E9PUF0, Q69ZX9

Related

ENSMUSP00000110158.3, ENSMUST00000114513.9

Conserved Domains (4) summary

sd00045
Location:562 → 588

ANK; ANK repeat [structural motif]

pfam12796
Location:562 → 652

Ank_2; Ankyrin repeats (3 copies)

pfam17959
Location:144 → 227

EF-hand_14; EF-hand domain

pfam04960
Location:249 → 535

Glutaminase
NM_001113383.1 → NP_001106854.1 glutaminase kidney isoform, mitochondrial isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) includes an alternate exon, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AK173039, BE849049

Consensus CDS

CCDS48256.1

UniProtKB/Swiss-Prot

D3Z7P3

Related

ENSMUSP00000110155.2, ENSMUST00000114510.8

Conserved Domains (1) summary

pfam04960
Location:249 → 535

Glutaminase; Glutaminase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

52202608..52274168 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017316236.2 → XP_017171725.1 glutaminase kidney isoform, mitochondrial isoform X4

Conserved Domains (3) summary

sd00045
Location:224 → 250

ANK; ANK repeat [structural motif]

pfam12796
Location:224 → 314

Ank_2; Ankyrin repeats (3 copies)

pfam04960
Location:9 → 197

Glutaminase
XM_036160625.1 → XP_036016518.1 glutaminase kidney isoform, mitochondrial isoform X2

UniProtKB/TrEMBL

D3Z7P4

Conserved Domains (3) summary

sd00045
Location:333 → 359

ANK; ANK repeat [structural motif]

pfam04960
Location:20 → 304

Glutaminase

pfam12796
Location:333 → 425

Ank_2; Ankyrin repeats (3 copies)
XM_006495702.3 → XP_006495765.1 glutaminase kidney isoform, mitochondrial isoform X2

See identical proteins and their annotated locations for XP_006495765.1

UniProtKB/TrEMBL

D3Z7P4

Related

ENSMUSP00000110157.2, ENSMUST00000114512.8

Conserved Domains (3) summary

sd00045
Location:333 → 359

ANK; ANK repeat [structural motif]

pfam04960
Location:20 → 304

Glutaminase

pfam12796
Location:333 → 425

Ank_2; Ankyrin repeats (3 copies)
XM_006495701.4 → XP_006495764.1 glutaminase kidney isoform, mitochondrial isoform X1

Conserved Domains (2) summary

pfam17959
Location:144 → 227

EF-hand_14; EF-hand domain

pfam04960
Location:249 → 535

Glutaminase
XM_006495704.5 → XP_006495767.1 glutaminase kidney isoform, mitochondrial isoform X3

Conserved Domains (2) summary

pfam17959
Location:144 → 227

EF-hand_14; EF-hand domain

cl00907
Location:249 → 353

Glutaminase