CETN2 centrin, EF-hand protein, 2 [Homo sapiens (human)] - Gene

Summary

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Official Symbol: CETN2provided by HGNC
Official Full Name: centrin, EF-hand protein, 2provided by HGNC
Primary source: HGNC:1867
See related: HPRD:02051; MIM:300006
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CALT; CEN2
Summary: Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome. [provided by RefSeq, Jul 2008]

Genomic context

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Location :: Xq28

Sequence :: Chromosome: X; NC_000023.10 (151995871..151999301, complement)

See CETN2 in Epigenomics, MapViewer

Chromosome X - NC_000023.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cen2 influences the binding of RPA and XPA with damaged DNA.
Title: Influence of centrin 2 on the interaction of nucleotide excision repair factors with damaged DNA.
xeroderma pigmentosum complementation group C expression correlates with a decreased amount of CENTRIN 2 transcript and protein
Title: Differential contribution of XPC, RAD23A, RAD23B and CENTRIN 2 to the UV-response in human cells.
The stability of centrin is regulated in part by Aurora A.
Title: Control of centrin stability by Aurora A.
Mps1-dependent phosphorylation of Cetn2 stimulates the canonical centriole assembly pathway.
Title: Mps1 phosphorylation sites regulate the function of centrin 2 in centriole assembly.
CDC25B forms a close association with Ctn-2 proteins at the centrosome.
Title: CDC25B associates with a centrin 2-containing complex and is involved in maintaining centrosome integrity.
oxidative radicals induce high proportions of irreversible damages (polymerisation) centrin 2 is highly sensitive to ionising radiation.
Title: Oxidative stress induces mainly human centrin 2 polymerisation.
Observational study of gene-disease association. (HuGE Navigator)
Title: Centrosome-related genes, genetic variation, and risk of breast cancer.
The structure of C-HsCen2 [the C-terminal domain of HsCen2 (T94-Y172)] in complex with R17-hSfi1-20 was determined.
Title: Structure, dynamics and thermodynamics of the human centrin 2/hSfi1 complex.
The nucleocytoplasmic shuttling of centrin-2 depends on the SUMO system.
Title: SUMO-dependent regulation of centrin-2.
lower centrin levels in oligoasthnozoospermic males resulted in lower pregnancy percentage in this group after ICSI.
Title: Human sperm centrin levels & outcome of intracytoplasmic sperm injection (ICSI)--a pilot study.

Submit: New GeneRIF Correction See all (22)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Gag, Pr55	gag	Studies using a combined approach of immunofluorescence detection of Gag protein and in situ hybridization detection of viral genomic RNA indicate Gag co-localizes with Psi+ RNA in the perinuclear region and also co-localizes with centrins	PubMed
Vpr, p15	vpr	HIV-1 Vpr-expressing HeLa cells stained with centriole markers pericentrin, gamma-tubulin, and Cen2 contain multiple pairs of centrioles scattered in the perinuclear region, indicating Vpr causes the accumulation of multiple centrosome-like structures	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
P41208	O60289	SFI1	HPRD	PubMed
P41208	Q01831	XPC	HPRD	PubMed
BioGRID:107496	BioGRID:106903	ARSA	BioGRID	PubMed	Two-hybrid
BioGRID:107496	BioGRID:125225	CDCA5	BioGRID	PubMed	Co-fractionation
BioGRID:107496	BioGRID:204930	Cetn2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107496	BioGRID:122921	NAA40	BioGRID	PubMed	Co-fractionation
BioGRID:107496	BioGRID:111456	POU5F1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107496	BioGRID:120469	PRMT6	BioGRID	PubMed	Two-hybrid
BioGRID:107496	BioGRID:111824	RAD23B	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107496	BioGRID:115153	SFI1	BioGRID	PubMed	Reconstituted Complex
BioGRID:107496	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107496	BioGRID:115939	USP39	BioGRID	PubMed	Co-fractionation
BioGRID:107496	BioGRID:123890	USP44	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107496	BioGRID:117382	USP49	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107496	BioGRID:113345	XPC	BioGRID	PubMed	Affinity Capture-Western; Co-fractionation; Co-purification; Reconstituted Complex

Pathways from BioSystems

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Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystem
Cell Cycle
Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
Centrosome maturation, organism-specific biosystem (from REACTOME)
Centrosome maturation, organism-specific biosystemThe centrosome is the primary microtubule organizing center (MTOC) in vertebrate cells and plays an important role in orchestrating the formation of the mitotic spindle. Centrosome maturation is an ...
G2/M Transition, organism-specific biosystem (from REACTOME)
G2/M Transition, organism-specific biosystemCyclin A can also form complexes with Cdc2 (Cdk1). Together with three B-type cyclins, Cdc2 (Cdk1) regulates the transition from G2 into mitosis. These complexes are activated by dephosphorylation of...
Loss of Nlp from mitotic centrosomes, organism-specific biosystem (from REACTOME)
Loss of Nlp from mitotic centrosomes, organism-specific biosystemDuring interphase, Nlp interacts with gamma-tubulin ring complexes (gamma-TuRC), and is thought to contribute to the organization of interphase microtubules (Casenghi et al.,2003). Plk1 is activated...
Loss of proteins required for interphase microtubule organizationÂ from the centrosome, organism-specific biosystem (from REACTOME)
Loss of proteins required for interphase microtubule organizationÂ from the centrosome, organism-specific biosystemIn addition to recruiting proteins and complexes necessary for increased microtubule nucleation, centrosomal maturation involves the loss of proteins involved in interphase microtubule organization ...
Mitotic G2-G2/M phases, organism-specific biosystem (from REACTOME)
Mitotic G2-G2/M phases, organism-specific biosystem
Mitotic G2-G2/M phases
Nucleotide excision repair, organism-specific biosystem (from KEGG)
Nucleotide excision repair, organism-specific biosystemNucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the ...
Nucleotide excision repair, conserved biosystem (from KEGG)
Nucleotide excision repair, conserved biosystemNucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the ...
Recruitment of mitotic centrosome proteins and complexes, organism-specific biosystem (from REACTOME)
Recruitment of mitotic centrosome proteins and complexes, organism-specific biosystemThe mitotic spindle becomes established once centrosomes have migrated to opposite poles and the nuclear envelope has broken down. During this stage, interphase centrosomes mature into mitotic centro...

General gene information

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Markers

DXS52 (e-PCR)
- UniSTS:98875

STS-X72964 (e-PCR)
- UniSTS:28396

Homology

Homologs of the CETN2 gene: The CETN2 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A.thaliana, and rice.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
ATP-dependent helicase activity	IEA Inferred from Electronic Annotation more info
G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
calcium ion binding	NAS Non-traceable Author Statement more info	PubMed
heterotrimeric G-protein binding	IEA Inferred from Electronic Annotation more info
microtubule binding	IEA Inferred from Electronic Annotation more info
nucleic acid binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
G2/M transition of mitotic cell cycle	TAS Traceable Author Statement more info
cell division	IEA Inferred from Electronic Annotation more info
centriole replication	IMP Inferred from Mutant Phenotype more info	PubMed
mitosis	NAS Non-traceable Author Statement more info	PubMed
mitotic cell cycle	TAS Traceable Author Statement more info
nucleotide-excision repair	IDA Inferred from Direct Assay more info	PubMed
regulation of cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
spermatogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
XPC complex	IDA Inferred from Direct Assay more info	PubMed
centriole	IDA Inferred from Direct Assay more info	PubMed
centrosome	IDA Inferred from Direct Assay more info	PubMed
centrosome	NAS Non-traceable Author Statement more info	PubMed
cytosol	TAS Traceable Author Statement more info
intracellular	IDA Inferred from Direct Assay more info
microtubule basal body	IEA Inferred from Electronic Annotation more info
photoreceptor connecting cilium	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: centrin-2

Names: centrin-2; caltractin (20kD calcium-binding protein)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_004344.1 → NP_004335.1 centrin-2

Status: REVIEWED

Source sequence(s)

X72964

Consensus CDS

CCDS14716.1

UniProtKB/Swiss-Prot

P41208

Conserved Domains (2) summary

cd00051
Location:105 – 167
Blast Score: 152

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

PTZ00183
Location:16 – 172
Blast Score: 510

PTZ00183; centrin; Provisional

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 PATCHES

Genomic

NW_003871103.2 Reference GRCh37.p10 PATCHES

Range

261310..264740, complement

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GenBank, FASTA, Sequence Viewer (Graphics)

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000023.10 Reference GRCh37.p10 Primary Assembly

Range

151995871..151999301, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018934.1 Alternate CHM1_1.0

Range

151862510..151865941, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AMYH01023250.1 (37789..41220)	None
genomic	AY919675.1	AAW82436.1
genomic	CH471172.2	EAW72900.1
genomic	U82671.5 (116086..119516)	None
mRNA	AK311940.1	BAG34881.1
mRNA	BC005334.1	AAH05334.1
mRNA	BC013873.2	AAH13873.1
mRNA	BT007256.1	AAP35920.1
mRNA	X72964.1	CAA51467.1
other-genetic	AM393039.1	CAL37917.1