Tpsab1 tryptase alpha/beta 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tpsab1provided by MGI
Official Full Name: tryptase alpha/beta 1provided by MGI
Primary source: MGI:MGI:96943
See related: Ensembl:ENSMUSG00000024173 AllianceGenome:MGI:96943
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mcp7; Mcp-7; Mcpt7; MMCP-7
Summary: Enables peptidase activity. Acts upstream of or within proteolysis. Located in extracellular space and mast cell granule. Is expressed in several structures, including alimentary system; brain; immune system; integumental system; and reproductive system. Orthologous to human TPSAB1 (tryptase alpha/beta 1) and TPSD1 (tryptase delta 1). [provided by Alliance of Genome Resources, Apr 2022]
Annotation information: Note: Genetic polymorphisms result in both protein coding and non-coding alleles of this gene.
Expression: Biased expression in stomach adult (RPKM 11.7), adrenal adult (RPKM 1.6) and 3 other tissues See more
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Genomic context

Location:: 17 A3.3; 17 12.53 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (25562219..25564536, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (25343245..25345562, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data indicate that the direct degradation of the integrin subunits by rmMCP-7 is sufficient to initiate angiogenesis. The results demonstrate, for the first time, that mMCP-7 acts in angiogenesis through integrin degradation.
Title: Mast Cell Protease 7 Promotes Angiogenesis by Degradation of Integrin Subunits.
Following TNFalpha treatment, increased elastase and tryptase modify the balance between activation and deactivation, elevating active chemerin concentration in adipocyte media and subsequent CMKLR1 activation.
Title: Elastase and tryptase govern TNFα-mediated production of active chemerin by adipocytes.
Because mMCP-6 and mMCP-7 can compensate for each other in this mouse disease model, the elimination of both tryptases is necessary to reveal the prominent roles of these serine proteases in joint inflammation and destruction.
Title: The mouse mast cell-restricted tetramer-forming tryptases mouse mast cell protease 6 and mouse mast cell protease 7 are critical mediators in inflammatory arthritis.
transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor
Title: Transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor.
TGF-beta stimulates mmcp-7 transcription through the Smad3-Smad4 pathway as well as c-fos induction
Title: Transcriptional regulation of mouse mast cell protease-7 by TGF-beta.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (1) 1 citation
Endonuclease-mediated (3)

General gene information

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Markers

Tpsab1 (e-PCR), detects polymorphism
- UniSTS:536696

Tpsab1 (e-PCR), detects polymorphism
- UniSTS:536697

M-04423 (e-PCR)
- UniSTS:186325

Mcpt7 (e-PCR), detects polymorphism
- UniSTS:143845

L00654 (e-PCR)
- UniSTS:180978

Mcpt7 (e-PCR), detects polymorphism
- UniSTS:265726

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of blood coagulation	TAS Traceable Author Statement more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in mast cell granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: tryptase

Names: mast cell protease 7; mast cell-restricted serine protease 7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031187.4 → NP_112464.4 tryptase precursor

See identical proteins and their annotated locations for NP_112464.4

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the protein-coding transcript expressed in BALB/c and CZECHII strains.

Source sequence(s)

L00653, L00654

UniProtKB/Swiss-Prot

Q02844

UniProtKB/TrEMBL

A1Z090, Q921N4

Conserved Domains (2) summary

smart00020
Location:29 → 265

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:29 → 266

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

RNA

NR_033521.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents the transcript expressed in C57BL/6, and uses a cryptic splice donor site compared to variant 1 (PMID: 8576265). C57BL/6 contains a G->A mutation that inactivates the normal splice donor site as used in variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC122454, U42405

Related

ENSMUST00000249719.1