Nceh1 neutral cholesterol ester hydrolase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nceh1provided by MGI
Official Full Name: neutral cholesterol ester hydrolase 1provided by MGI
Primary source: MGI:MGI:2443191
See related: Ensembl:ENSMUSG00000027698 AllianceGenome:MGI:2443191
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nceh; CPO-BP; Aadacl1; B230106I24Rik
Summary: Enables phosphate ion binding activity and serine hydrolase activity. Involved in ether lipid metabolic process. Acts upstream of or within SMAD protein signal transduction; protein dephosphorylation; and xenobiotic metabolic process. Located in membrane. Is expressed in nervous system; retina; and tooth. Orthologous to human NCEH1 (neutral cholesterol ester hydrolase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in kidney adult (RPKM 41.2), adrenal adult (RPKM 26.8) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 A3; 3 10.82 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (27237153..27299112)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (27183004..27245266)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results suggest that Nceh1 plays a dominant role over Lipe in the hydrolysis of CE and subsequent cholesterol efflux in MPMs
Title: Critical role of neutral cholesteryl ester hydrolase 1 in cholesteryl ester hydrolysis in murine macrophages.
reducing the cholesteryl ester content of foam cells, Nceh1 may protect against the pro-apoptotic effect of oxysterols and modulate the development of atherosclerosis.
Title: Absence of Nceh1 augments 25-hydroxycholesterol-induced ER stress and apoptosis in macrophages.
Gender difference of atherogenesis is partly accounted for activation of neutral cholesterol ester hydrolase through estrogen-dependent translocation of A-kinase type II in macrophages.
Title: Estrogen-dependent activation of neutral cholesterol ester hydrolase underlying gender difference of atherogenesis in apoE-/- mice.
Nceh1 is involved in the adrenal cholesterol metabolism, and the cholesterol ester hydrolytic activity in adrenal glands is associated with the organ enlargement.
Title: Abrogation of neutral cholesterol ester hydrolytic activity causes adrenal enlargement.
NCEH is targeted to the ER of macrophages, where it hydrolyzes CE to deliver cholesterol for efflux out of the cells.
Title: Targeting of neutral cholesterol ester hydrolase to the endoplasmic reticulum via its N-terminal sequence.
Genetic ablation of Nceh1 promotes foam cell formation and the development of atherosclerosis in mice.
Title: Ablation of neutral cholesterol ester hydrolase 1 accelerates atherosclerosis.
NCEH is responsible for a major part of nCEH activity in macrophages and may be a potential therapeutic target for the prevention of atherosclerosis.
Title: Identification of neutral cholesterol ester hydrolase, a key enzyme removing cholesterol from macrophages.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D3Mit305 (e-PCR), detects polymorphism
- UniSTS:129741

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylalkylglycerol acetylhydrolase activity	IEA Inferred from Electronic Annotation more info
enables carboxylic ester hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables phosphate ion binding	IDA Inferred from Direct Assay more info	PubMed
enables serine hydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine hydrolase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ether lipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within xenobiotic metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: neutral cholesterol ester hydrolase 1

Names: 2-acetyl MAGE hydrolase; acetylalkylglycerol acetylhydrolase; arylacetamide deacetylase-like 1; chlorpyrifos oxon-binding protein

NP_848887.1

EC 3.1.1.71

XP_011247986.1

EC 3.1.1.71

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_178772.4 → NP_848887.1 neutral cholesterol ester hydrolase 1

See identical proteins and their annotated locations for NP_848887.1

Status: VALIDATED

Source sequence(s)

AC121099, AC157919

Consensus CDS

CCDS17271.1

UniProtKB/Swiss-Prot

Q69ZL0, Q8BLF1, Q8BZK3

Related

ENSMUSP00000045864.9, ENSMUST00000046515.15

Conserved Domains (2) summary

COG0657
Location:73 → 385

Aes; Acetyl esterase/lipase [Lipid transport and metabolism]

pfam07859
Location:109 → 382

Abhydrolase_3; alpha/beta hydrolase fold

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

27237153..27299112

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011249684.2 → XP_011247986.1 neutral cholesterol ester hydrolase 1 isoform X1

Conserved Domains (1) summary

cl21494
Location:1 → 248

Abhydrolase; alpha/beta hydrolases