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    Atp12a ATPase H+/K+ transporting non-gastric alpha2 subunit [ Rattus norvegicus (Norway rat) ]

    Gene ID: 171028, updated on 13-Apr-2024

    Summary

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    Official Symbol
    Atp12aprovided by RGD
    Official Full Name
    ATPase H+/K+ transporting non-gastric alpha2 subunitprovided by RGD
    Primary source
    RGD:620569
    See related
    Ensembl:ENSRNOG00000020685 AllianceGenome:RGD:620569
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    Contributes to P-type potassium:proton transporter activity. Involved in response to metal ion and response to organic cyclic compound. Located in apical plasma membrane. Biomarker of hypokalemia. Orthologous to human ATP12A (ATPase H+/K+ transporting non-gastric alpha2 subunit). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Kidney (RPKM 21.2), Uterus (RPKM 5.7) and 2 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Atp12a in Genome Data Viewer
    Location:
    15p12
    Exon count:
    23
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 15 NC_086033.1 (34559209..34583866)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 15 NC_051350.1 (30443571..30468229)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 15 NC_005114.4 (36561306..36590171)

    Chromosome 15 - NC_086033.1Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA SNORA17 Neighboring gene granzyme B Neighboring gene ring finger protein 17 Neighboring gene TM2 domain-containing protein 1 pseudogene Neighboring gene centromere protein J

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats. Sárközy M, et al. Cardiovasc Diabetol, 2013 Jan 15. PMID 23320804, Free PMC Article
    2. Expression of HKalpha2 protein is increased selectively in renal medulla by chronic hypokalemia. Codina J, et al. Am J Physiol, 1998 Sep. PMID 9729517
    3. A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia. Kone BC, et al. J Biol Chem, 1998 Jan 30. PMID 9446555
    4. Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus. Crowson MS, et al. J Biol Chem, 1992 Jul 5. PMID 1320029
    5. Conversion of the low affinity ouabain-binding site of non-gastric H,K-ATPase into a high affinity binding site by substitution of only five amino acids. Qiu LY, et al. J Biol Chem, 2006 May 12. PMID 16531406

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The substitution of 5 amino acids in the low affinity ouabain binding site of non-gastric proton-potassium exchanging ATPase in order to increase ouabain binding affinity is reported.
      Title: Conversion of the low affinity ouabain-binding site of non-gastric H,K-ATPase into a high affinity binding site by substitution of only five amino acids.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to P-type potassium:proton transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables P-type potassium:proton transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables P-type potassium:proton transporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables P-type sodium:potassium-exchanging transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables P-type sodium:potassium-exchanging transporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables P-type sodium:potassium-exchanging transporter activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in establishment or maintenance of transmembrane electrochemical gradient IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intracellular potassium ion homeostasis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular sodium ion homeostasis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within potassium ion homeostasis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in potassium ion import across plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in proton transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of pH IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of pH ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in response to metal ion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to organic cyclic compound IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in sodium ion export across plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in actin cytoskeleton ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in apical plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in apical plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in basolateral plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in basolateral plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    potassium-transporting ATPase alpha chain 2
    Names
    ATPase, H+/K+ transporting, nongastric, alpha polypeptide
    H-K-ATPase alpha 2
    HK alpha 2
    hydrogen/potassium-exchanging ATPase 12A
    non-gastric H(+)/K(+) ATPase subunit alpha
    non-gastric Na(+)/K(+) ATPase subunit alpha
    proton pump
    sodium pump
    NP_001288593.1
    NP_598201.2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001301664.1 → NP_001288593.1  potassium-transporting ATPase alpha chain 2 isoform b

      See identical proteins and their annotated locations for NP_001288593.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) has an alternate 5' sequence, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (b, also known as HKalpha2b) has a shorter N-terminus, compared to isoform a.
      Source sequence(s)
      JAXUCZ010000015
      UniProtKB/Swiss-Prot
      P54708
      UniProtKB/TrEMBL
      A6KH96
      Conserved Domains (5) summary
      TIGR01106
      Location:1 → 928
      ATPase-IIC_X-K; sodium or proton efflux -- potassium uptake antiporter, P-type ATPase, alpha subunit
      pfam00122
      Location:38 → 269
      E1-E2_ATPase; E1-E2 ATPase
      pfam00689
      Location:703 → 913
      Cation_ATPase_C; Cation transporting ATPase, C-terminus
      pfam12710
      Location:505 → 630
      HAD; haloacid dehalogenase-like hydrolase
      pfam13246
      Location:331 → 425
      Cation_ATPase; Cation transport ATPase (P-type)

    2. NM_133517.2 → NP_598201.2  potassium-transporting ATPase alpha chain 2 isoform a

      See identical proteins and their annotated locations for NP_598201.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longer isoform (a, also known as HKalpha2a).
      Source sequence(s)
      JAXUCZ010000015
      UniProtKB/Swiss-Prot
      P54708
      UniProtKB/TrEMBL
      A6KH95, G3V8S4
      Related
      ENSRNOP00000028093.3, ENSRNOT00000028093.5
      Conserved Domains (6) summary
      smart00831
      Location:53 → 124
      Cation_ATPase_N; Cation transporter/ATPase, N-terminus
      TIGR01106
      Location:40 → 1036
      ATPase-IIC_X-K; sodium or proton efflux -- potassium uptake antiporter, P-type ATPase, alpha subunit
      pfam00122
      Location:146 → 377
      E1-E2_ATPase; E1-E2 ATPase
      pfam00689
      Location:811 → 1021
      Cation_ATPase_C; Cation transporting ATPase, C-terminus
      pfam12710
      Location:613 → 738
      HAD; haloacid dehalogenase-like hydrolase
      pfam13246
      Location:439 → 533
      Cation_ATPase; Cation transport ATPase (P-type)

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086033.1 Reference GRCr8

      Range
      34559209..34583866
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P54708.1 GenPept UniProtKB/Swiss-Prot:P54708

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