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    UBE2J2 ubiquitin conjugating enzyme E2 J2 [ Homo sapiens (human) ]

    Gene ID: 118424, updated on 5-Mar-2024

    Summary

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    Official Symbol
    UBE2J2provided by HGNC
    Official Full Name
    ubiquitin conjugating enzyme E2 J2provided by HGNC
    Primary source
    HGNC:HGNC:19268
    See related
    Ensembl:ENSG00000160087 MIM:619756; AllianceGenome:HGNC:19268
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    UBC6; NCUBE2; NCUBE-2; PRO2121
    Summary
    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
    Expression
    Broad expression in testis (RPKM 15.7), fat (RPKM 6.0) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    1p36.33
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (1253912..1273854, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (682910..702871, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (1189292..1209234, complement)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1167335-1168016 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1168017-1168698 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:1169483-1169984 Neighboring gene beta-1,3-galactosyltransferase 6 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1182045-1182826 Neighboring gene C1q and TNF related 12 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1182827-1183607 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 14 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr1:1199775-1200974 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1207366-1207874 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1207875-1208381 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1208382-1208889 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 49 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 50 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:1210080-1210718 Neighboring gene long intergenic non-protein coding RNA 1786 Neighboring gene MPRA-validated peak7 silencer Neighboring gene sodium channel epithelial 1 subunit delta Neighboring gene CEB15 minisatellite repeat instability region Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1223369-1223916 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1223917-1224463 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:1224736-1225308 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 51 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:1227773-1228318 Neighboring gene ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 Neighboring gene microRNA 6726

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. The human ubiquitin conjugating enzyme UBE2J2 (Ubc6) is a substrate for proteasomal degradation. Lam SY, et al. Biochem Biophys Res Commun, 2014 Aug 29. PMID 25083800
    2. Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis. Tan JME, et al. Atherosclerosis, 2019 Feb. PMID 30658189
    3. UBE2J2 promotes hepatocellular carcinoma cell epithelial-mesenchymal transition and invasion in vitro. Chen S, et al. Oncotarget, 2017 Sep 22. PMID 29069742, Free PMC Article
    4. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. van de Weijer ML, et al. J Cell Sci, 2017 Sep 1. PMID 28743740, Free PMC Article
    5. MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins. Stefanovic-Barrett S, et al. EMBO Rep, 2018 May. PMID 29519897, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. UBE2J2 as an important partner of MARCH6 for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis.
      Title: Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis.
    2. UBE2G2 was crucial for the degradation of various immunoreceptors. UBE2J2, on the other hand, counteracted US2-induced endoplasmic reticulum-associated protein degradation by downregulating TRC8 expression.
      Title: Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation.
    3. Endoplasmic reticulum localization and cis-enzymatic activity play a role in regulating the steady state expression of human Ube2J2, ER localization and cis-enzymatic activity play a role in regulating the steady state expression of human Ube2J2.
      Title: The human ubiquitin conjugating enzyme UBE2J2 (Ubc6) is a substrate for proteasomal degradation.
    4. analysis of involvement of E2 enzymes in specifying polyubiquitin chain assembly
      Title: The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin conjugating enzyme activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin conjugating enzyme activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ERAD pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ERAD pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of protein targeting to mitochondrion HMP PubMed 
    involved_in protein K6-linked ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein polyubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in response to unfolded protein IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  
    is_active_in endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum membrane TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of ubiquitin ligase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ubiquitin-conjugating enzyme E2 J2
    Names
    E2 ubiquitin-conjugating enzyme J2
    non-canonical ubiquitin-conjugating enzyme 2
    ubiquitin conjugating enzyme 6
    ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
    yeast UBC6 homolog
    NP_477515.2
    NP_919296.1
    NP_919439.1
    NP_919440.1
    XP_005244775.1
    XP_005244776.1
    XP_006710396.1
    XP_011538915.1
    XP_011538916.1
    XP_016855728.1
    XP_047300282.1
    XP_047300295.1
    XP_047300312.1
    XP_047300314.1
    XP_054190186.1
    XP_054190187.1
    XP_054190188.1
    XP_054190189.1
    XP_054190190.1
    XP_054190191.1
    XP_054190192.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_058167.3 → NP_477515.2  ubiquitin-conjugating enzyme E2 J2 isoform 2

      See identical proteins and their annotated locations for NP_477515.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an in-frame internal exon, as compared to variant 1. The encoded isoform (2) is thus missing an internal segment, as compared to isoform 1.
      Source sequence(s)
      AK056065, AK098803, BE515187, BG499172, BG717336, BQ881730
      Consensus CDS
      CCDS14.1
      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84
      Related
      ENSP00000305826.7, ENST00000349431.11
      Conserved Domains (1) summary
      cd00195
      Location:14 → 127
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    2. NM_194315.2 → NP_919296.1  ubiquitin-conjugating enzyme E2 J2 isoform 1

      See identical proteins and their annotated locations for NP_919296.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AK098803, AW411508, BE382927, BE515187, BG717336, BM544827
      Consensus CDS
      CCDS15.1
      UniProtKB/Swiss-Prot
      Q8N2K1
      Related
      ENSP00000383719.4, ENST00000400930.8
      Conserved Domains (1) summary
      cd00195
      Location:14 → 143
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    3. NM_194457.2 → NP_919439.1  ubiquitin-conjugating enzyme E2 J2 isoform 3

      See identical proteins and their annotated locations for NP_919439.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) lacks two consecutive internal exons, as compared to variant 1. It uses a downstream start codon, so the encoded isoform (3) has a shorter N-terminus, as compared to isoform 1.
      Source sequence(s)
      AK098803, BE515187, BG112933, BG717336, BM765278
      Consensus CDS
      CCDS16.1
      UniProtKB/TrEMBL
      A0A024R0A7, A0A9K3Y7P8, A6NGS0
      Related
      ENSP00000383718.2, ENST00000400929.6
      Conserved Domains (1) summary
      cd00195
      Location:2 → 75
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    4. NM_194458.2 → NP_919440.1  ubiquitin-conjugating enzyme E2 J2 isoform 3

      See identical proteins and their annotated locations for NP_919440.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) has two alternate splice sites in the 5' region, as compared to variant 1. It uses a downstream start codon, so the encoded isoform (3) has a shorter N-terminus, as compared to isoform 1.
      Source sequence(s)
      AK075017, AK098803, BE515187, BG717336, BM926567
      Consensus CDS
      CCDS16.1
      UniProtKB/TrEMBL
      A0A024R0A7, A0A9K3Y7P8, A6NGS0
      Related
      ENSP00000344857.2, ENST00000347370.6
      Conserved Domains (1) summary
      cd00195
      Location:2 → 75
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      1253912..1273854 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047444339.1 → XP_047300295.1  ubiquitin-conjugating enzyme E2 J2 isoform X5

    2. XM_005244719.5 → XP_005244776.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      See identical proteins and their annotated locations for XP_005244776.1

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84
      Related
      ENSP00000353653.2, ENST00000360466.6
      Conserved Domains (1) summary
      cd00195
      Location:14 → 127
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    3. XM_005244718.4 → XP_005244775.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      See identical proteins and their annotated locations for XP_005244775.1

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84
      Conserved Domains (1) summary
      cd00195
      Location:14 → 127
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    4. XM_011540614.3 → XP_011538916.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      See identical proteins and their annotated locations for XP_011538916.1

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84
      Conserved Domains (1) summary
      cd00195
      Location:14 → 127
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    5. XM_006710333.4 → XP_006710396.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      See identical proteins and their annotated locations for XP_006710396.1

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84
      Conserved Domains (1) summary
      cd00195
      Location:14 → 127
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    6. XM_047444356.1 → XP_047300312.1  ubiquitin-conjugating enzyme E2 J2 isoform X5

    7. XM_011540613.4 → XP_011538915.1  ubiquitin-conjugating enzyme E2 J2 isoform X3

      See identical proteins and their annotated locations for XP_011538915.1

      UniProtKB/Swiss-Prot
      Q8N2K1
      Conserved Domains (1) summary
      cd00195
      Location:10 → 92
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    8. XM_047444358.1 → XP_047300314.1  ubiquitin-conjugating enzyme E2 J2 isoform X5

    9. XM_047444326.1 → XP_047300282.1  ubiquitin-conjugating enzyme E2 J2 isoform X1

    10. XM_017000239.3 → XP_016855728.1  ubiquitin-conjugating enzyme E2 J2 isoform X4

      UniProtKB/TrEMBL
      A0A024R0A7, A0A9K3Y7P8, A6NGS0
      Conserved Domains (1) summary
      cd00195
      Location:2 → 75
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      682910..702871 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054334213.1 → XP_054190188.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84

    2. XM_054334211.1 → XP_054190186.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84

    3. XM_054334217.1 → XP_054190192.1  ubiquitin-conjugating enzyme E2 J2 isoform X5

    4. XM_054334212.1 → XP_054190187.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84

    5. XM_054334214.1 → XP_054190189.1  ubiquitin-conjugating enzyme E2 J2 isoform X2

      UniProtKB/Swiss-Prot
      A8MYC7, Q504T9, Q8N2K1, Q96N26, Q96T84

    6. XM_054334215.1 → XP_054190190.1  ubiquitin-conjugating enzyme E2 J2 isoform X3

    7. XM_054334216.1 → XP_054190191.1  ubiquitin-conjugating enzyme E2 J2 isoform X4

      UniProtKB/TrEMBL
      A0A024R0A7, A0A9K3Y7P8, A6NGS0

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_194316.1: Suppressed sequence

      Description
      NM_194316.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8N2K1.3 GenPept UniProtKB/Swiss-Prot:Q8N2K1

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