Sp5 trans-acting transcription factor 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sp5provided by MGI
Official Full Name: trans-acting transcription factor 5provided by MGI
Primary source: MGI:MGI:1927715
See related: Ensembl:ENSMUSG00000075304 AllianceGenome:MGI:1927715
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Acts upstream of or within bone morphogenesis; cellular response to organic cyclic compound; and post-anal tail morphogenesis. Predicted to be located in nucleus. Is expressed in several structures, including alimentary system; brain; branchial arch; embryo ectoderm; and embryo mesenchyme. Orthologous to human SP5 (Sp5 transcription factor). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in duodenum adult (RPKM 49.5), small intestine adult (RPKM 18.4) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 C2; 2 41.49 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (70305267..70308070)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (70474923..70477726)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deficiency of TRPM2 leads to embryonic neurogenesis defects in hyperthermia.
Title: Deficiency of TRPM2 leads to embryonic neurogenesis defects in hyperthermia.
Functional studies revealed that knockdown of Nanog eliminated the mESC self-renewal-promoting ability of Sp5. Finally, we demonstrated that the self-renewal-promoting function of Sp5 was largely dependent on its zinc finger domains
Title: Sp5 induces the expression of Nanog to maintain mouse embryonic stem cell self-renewal.
Sp5 is able to convert mouse epiblast stem cells into a naive pluripotent state.
Title: Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal.
Sp5/8 bind directly to GC boxes in Wnt target gene enhancers and to adjacent, or distally positioned, chromatin-bound T-cell factor (Tcf) 1/lymphoid enhancer factor (Lef) 1 to facilitate recruitment of beta-catenin to target gene enhancers.
Title: Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription.
Sp5 and Sp8 are pivotal downstream effectors of Wnt3a/beta-catenin that are essential for the self-renewal and differentiation of neuromesodermal stem cells.
Title: Transcriptional profiling of Wnt3a mutants identifies Sp transcription factors as essential effectors of the Wnt/β-catenin pathway in neuromesodermal stem cells.
Sp5 transcription factor mediates the downstream responses to Wnt/beta-catenin signaling by directly repressing Sp1 target genes
Title: Wnt-mediated down-regulation of Sp1 target genes by a transcriptional repressor Sp5.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

General gene information

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Markers

NoName (e-PCR)
- UniSTS:493350

Sp5 (e-PCR), detects polymorphism
- UniSTS:186544

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within bone morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to organic cyclic compound	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within post-anal tail morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcription factor Sp5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022435.2 → NP_071880.1 transcription factor Sp5

See identical proteins and their annotated locations for NP_071880.1

Status: PROVISIONAL

Source sequence(s)

AK036234

Consensus CDS

CCDS16106.1

UniProtKB/Swiss-Prot

Q149T2, Q9JHX2

Related

ENSMUSP00000097620.3, ENSMUST00000100043.3

Conserved Domains (5) summary

COG5048
Location:311 → 384

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:301 → 320

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:342 → 365

zf-H2C2_2; Zinc-finger double domain

cd22541
Location:106 → 297

SP5_N; N-terminal domain of transcription factor Specificity Protein (SP) 5

cl41773
Location:31 → 47

SP1-4_N; N-terminal domain of transcription factor Specificity Proteins (SP) 1-4