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    Uba3 ubiquitin-like modifier activating enzyme 3 [ Mus musculus (house mouse) ]

    Gene ID: 22200, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Uba3provided by MGI
    Official Full Name
    ubiquitin-like modifier activating enzyme 3provided by MGI
    Primary source
    MGI:MGI:1341217
    See related
    Ensembl:ENSMUSG00000030061 AllianceGenome:MGI:1341217
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Ube1c; A830034N06Rik
    Summary
    The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
    Expression
    Broad expression in CNS E11.5 (RPKM 17.1), placenta adult (RPKM 15.3) and 24 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    6 D3; 6 44.9 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (97160593..97182608, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (97183632..97205647, complement)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene EGF domain specific O-linked N-acetylglucosamine transferase Neighboring gene STARR-seq mESC enhancer starr_16775 Neighboring gene STARR-seq mESC enhancer starr_16776 Neighboring gene TATA element modulatory factor 1 Neighboring gene predicted gene, 26175 Neighboring gene STARR-positive B cell enhancer ABC_E2796 Neighboring gene STARR-seq mESC enhancer starr_16779 Neighboring gene STARR-positive B cell enhancer ABC_E1324 Neighboring gene STARR-seq mESC enhancer starr_16780 Neighboring gene ADP-ribosylation factor-like 6 interacting protein 5 Neighboring gene leiomodin 3 (fetal) Neighboring gene predicted gene, 32191

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Myeloid neddylation targets IRF7 and promotes host innate immunity against RNA viruses. Zhao M, et al. PLoS Pathog, 2021 Sep. PMID 34506605, Free PMC Article
    2. Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection. Cheng Q, et al. PLoS Pathog, 2018 Nov. PMID 30462731, Free PMC Article
    3. The cell cycle of early mammalian embryos: lessons from genetic mouse models. Artus J, et al. Cell Cycle, 2006 Mar. PMID 16481745
    4. Neddylation is required for herpes simplex virus type I (HSV-1)-induced early phase interferon-beta production. Zhang X, et al. Cell Mol Immunol, 2016 Sep. PMID 27593482, Free PMC Article
    5. Hepatic neddylation targets and stabilizes electron transfer flavoproteins to facilitate fatty acid β-oxidation. Zhang X, et al. Proc Natl Acad Sci U S A, 2020 Feb 4. PMID 31941714, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Transgenic (1) 
    • Gene trapped (1) 
    • Targeted (2)  1 citation
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NEDD8 activating enzyme activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables NEDD8 activating enzyme activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to NEDD8 activating enzyme activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NEDD8 activating enzyme activity ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables NEDD8 activating enzyme activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nuclear receptor binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein heterodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein-containing complex binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin-like modifier activating enzyme activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within endomitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein neddylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein neddylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within regulation of cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of protein-containing complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    NEDD8-activating enzyme E1 catalytic subunit
    Names
    NEDD8-activating enzyme E1C
    ubiquitin-activating enzyme 3
    ubiquitin-activating enzyme E1C
    NP_001104576.1
    NP_001288786.1
    NP_001288787.1
    NP_001288788.1
    NP_035796.2
    XP_011239592.1
    XP_030111175.1
    XP_030111176.1
    XP_036021915.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001111106.2 → NP_001104576.1  NEDD8-activating enzyme E1 catalytic subunit isoform 2

      See identical proteins and their annotated locations for NP_001104576.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.
      Source sequence(s)
      AK159381, AK166655, BY639644
      Consensus CDS
      CCDS51859.1
      UniProtKB/TrEMBL
      Q3TL72, Q66JT6
      Related
      ENSMUSP00000130954.2, ENSMUST00000164744.8
      Conserved Domains (3) summary
      cd01488
      Location:57 → 354
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam00899
      Location:53 → 352
      ThiF; ThiF family
      pfam08825
      Location:362 → 447
      E2_bind; E2 binding domain

    2. NM_001301857.1 → NP_001288786.1  NEDD8-activating enzyme E1 catalytic subunit isoform 3

      See identical proteins and their annotated locations for NP_001288786.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AK032514, AK159381, AK166655, BY639644
      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (2) summary
      cd01488
      Location:50 → 347
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam08825
      Location:355 → 440
      E2_bind; E2 binding domain

    3. NM_001301858.1 → NP_001288787.1  NEDD8-activating enzyme E1 catalytic subunit isoform 4

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes an isoform (4) that is shorter compared to isoform 1.
      Source sequence(s)
      AK159381, AK166655, BC080776, BY639644
      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (3) summary
      cd01488
      Location:62 → 341
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam00899
      Location:62 → 339
      ThiF; ThiF family
      pfam08825
      Location:349 → 434
      E2_bind; E2 binding domain

    4. NM_001301859.1 → NP_001288788.1  NEDD8-activating enzyme E1 catalytic subunit isoform 5

      Status: REVIEWED

      Description
      Transcript Variant: This variant (5) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AK014433, AK159381, CF928750
      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (2) summary
      cd01488
      Location:71 → 368
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam00899
      Location:67 → 366
      ThiF; ThiF family

    5. NM_011666.3 → NP_035796.2  NEDD8-activating enzyme E1 catalytic subunit isoform 1

      See identical proteins and their annotated locations for NP_035796.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AK159381, AK166655, BY639644
      Consensus CDS
      CCDS51860.1
      UniProtKB/Swiss-Prot
      O88598, Q3TG68, Q8C878, Q9D6B7
      UniProtKB/TrEMBL
      Q66JT6
      Related
      ENSMUSP00000086701.6, ENSMUST00000089287.7
      Conserved Domains (3) summary
      cd01488
      Location:71 → 368
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam00899
      Location:67 → 366
      ThiF; ThiF family
      pfam08825
      Location:376 → 461
      E2_bind; E2 binding domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      97160593..97182608 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030255316.2 → XP_030111176.1  NEDD8-activating enzyme E1 catalytic subunit isoform X2

      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (2) summary
      cd01488
      Location:48 → 327
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam08825
      Location:335 → 420
      E2_bind; E2 binding domain

    2. XM_030255315.2 → XP_030111175.1  NEDD8-activating enzyme E1 catalytic subunit isoform X1

      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (2) summary
      cd01488
      Location:50 → 347
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam08825
      Location:355 → 440
      E2_bind; E2 binding domain

    3. XM_036166022.1 → XP_036021915.1  NEDD8-activating enzyme E1 catalytic subunit isoform X1

      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (2) summary
      cd01488
      Location:50 → 347
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam08825
      Location:355 → 440
      E2_bind; E2 binding domain

    4. XM_011241290.4 → XP_011239592.1  NEDD8-activating enzyme E1 catalytic subunit isoform X1

      See identical proteins and their annotated locations for XP_011239592.1

      UniProtKB/TrEMBL
      Q66JT6
      Conserved Domains (2) summary
      cd01488
      Location:50 → 347
      Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...
      pfam08825
      Location:355 → 440
      E2_bind; E2 binding domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8C878.2 GenPept UniProtKB/Swiss-Prot:Q8C878

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