Phactr4 phosphatase and actin regulator 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Phactr4provided by MGI
Official Full Name: phosphatase and actin regulator 4provided by MGI
Primary source: MGI:MGI:2140327
See related: Ensembl:ENSMUSG00000066043 AllianceGenome:MGI:2140327
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA4120; 3110001B12Rik; C330013F19Rik
Summary: Enables actin binding activity; protein phosphatase 1 binding activity; and protein phosphatase activator activity. Involved in several processes, including enteric nervous system development; morphogenesis of embryonic epithelium; and negative regulation of integrin-mediated signaling pathway. Located in lamellipodium. Is expressed in central nervous system and retina. Orthologous to human PHACTR4 (phosphatase and actin regulator 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 6.0), limb E14.5 (RPKM 5.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 D2.3; 4 65.24 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (132083233..132149765, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (132355922..132422446, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Estrogen receptor beta increases clear cell renal cell carcinoma stem cell phenotype via altering the circPHACTR4/miR-34b-5p/c-Myc signaling.
Title: Estrogen receptor beta increases clear cell renal cell carcinoma stem cell phenotype via altering the circPHACTR4/miR-34b-5p/c-Myc signaling.
The Phactr4 forms functional associations with intermediate filament networks in adult neural stem cells.
Title: Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes.
Data discloses the central role of Phactr4 in a feedback loop, where actin monomers regulate their own number via the activation of a key regulator of actin dynamics.
Title: Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool.
This study demonistrated that the mRNA expression of phactr4 was progressively increased in the injury penumbra.
Title: Different expression patterns of Phactr family members in normal and injured mouse brain.
hactr4 negatively regulates integrin signaling through the RHO/ROCK pathway and coordinates protein phosphatase 1 (PP1) with cofilin activity to regulate cytoskeletal dynamics
Title: Phactr4 regulates directional migration of enteric neural crest through PP1, integrin signaling, and cofilin activity.
Phactr4 is critical for the spatially and temporally regulated transition in proliferation through differential regulation of PP1 and the cell cycle during neurulation and eye development
Title: Phactr4 regulates neural tube and optic fissure closure by controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125068 (e-PCR)
- UniSTS:161890

AW495572 (e-PCR)
- UniSTS:179076

AI527228 (e-PCR)
- UniSTS:164263

RH135880 (e-PCR)
- UniSTS:209878

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase 1 binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein phosphatase inhibitor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in closure of optic fissure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in enteric nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of integrin-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
involved_in neural crest cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in neural crest cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neural tube closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of catalytic activity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphatase and actin regulator 4

Names: humdy; protein Humpty dumpty

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001161797.2 → NP_001155269.1 phosphatase and actin regulator 4 isoform 2

Status: VALIDATED

Source sequence(s)

AL805897

Consensus CDS

CCDS51318.1

UniProtKB/Swiss-Prot

Q501J7

Related

ENSMUSP00000081185.6, ENSMUST00000084170.12

Conserved Domains (1) summary

smart00707
Location:549 → 573

RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2
NM_175306.5 → NP_780515.2 phosphatase and actin regulator 4 isoform 1

See identical proteins and their annotated locations for NP_780515.2

Status: VALIDATED

Source sequence(s)

AL805897

Consensus CDS

CCDS18724.1

UniProtKB/Swiss-Prot

A2ALF7, A2ALG0, F6QNZ3, Q501J7, Q5DTM4, Q6DI97, Q8C7U9

Related

ENSMUSP00000099628.4, ENSMUST00000102568.10

Conserved Domains (2) summary

smart00707
Location:576 → 600

RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2

pfam02755
Location:64 → 86

RPEL; RPEL repeat

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

132083233..132149765 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036163538.1 → XP_036019431.1 phosphatase and actin regulator 4 isoform X3

Conserved Domains (1) summary

smart00707
Location:491 → 515

RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2
XM_006538456.5 → XP_006538519.1 phosphatase and actin regulator 4 isoform X1

UniProtKB/Swiss-Prot

Q501J7

Related

ENSMUSP00000081270.5, ENSMUST00000084249.11

Conserved Domains (2) summary

smart00707
Location:586 → 610

RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2

pfam02755
Location:75 → 97

RPEL; RPEL repeat
XM_006538457.5 → XP_006538520.1 phosphatase and actin regulator 4 isoform X2

Conserved Domains (1) summary

smart00707
Location:559 → 583

RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2
XM_011250172.2 → XP_011248474.1 phosphatase and actin regulator 4 isoform X5

Conserved Domains (1) summary

smart00707
Location:302 → 326

RPEL; Repeat in Drosophila CG10860, human KIAA0680 and C. elegans F26H9.2
XM_036163539.1 → XP_036019432.1 phosphatase and actin regulator 4 isoform X4

Conserved Domains (1) summary

pfam02755
Location:75 → 97

RPEL; RPEL repeat