BRR2 ATP-dependent RNA helicase BRR2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: BRR2
Gene description: ATP-dependent RNA helicase BRR2
Primary source: SGD:S000000974
Locus tag: YER172C
See related: AllianceGenome:SGD:S000000974
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PRP44; RSS1; SLT22; SNU246
Summary: Enables RNA helicase activity. Involved in spliceosome conformational change to release U4 (or U4atac) and U1 (or U11). Located in nucleus. Part of U4/U6 x U5 tri-snRNP complex and U5 snRNP. Used to study retinitis pigmentosa and retinitis pigmentosa 33. Human ortholog(s) of this gene implicated in retinitis pigmentosa and retinitis pigmentosa 33. Orthologous to human SNRNP200 (small nuclear ribonucleoprotein U5 subunit 200). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See BRR2 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (529530..536021, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that the truncation of the first 247 amino acids of the N terminus in Bad Response to Refrigeration 2 (Delta247-Brr2) weakened RNA binding, reduced helicase activity, and reduced ATPase activity compared with wild type Brr2.
Title: Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity.
the N-terminal region autoinhibits Brr2 via substrate competition and conformational clamping
Title: The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation.
The N-terminal domain iscritical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its interaction with snRNAs and other spliceosomal proteins, revealing a role of Brr2 in spliceosomal activation in addition to U4/U6 unwinding.
Title: Brr2 plays a role in spliceosomal activation in addition to U4/U6 unwinding.
We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP.
Title: The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.
Sad1 maintains the integrity of the tri-snRNP by counteracting Brr2-mediated dissociation of tri-snRNP.
Title: Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis.
structure explains why Aar2 and Brr2 are mutually exclusive and provides important insights into the assembly of U5 snRNP
Title: Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.
the mechanism whereby Brr2 unwinds U4/U6 and show how this activity is potentially regulated prior to spliceosome activation
Title: The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA.
function for Brr2p in driving conformational rearrangements that lead to competence for the second step of splicing
Title: Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning.
Results suggest that Brr2 shares a similar helicase mechanism with Hel308, and demonstrate that Hel308-II interacts with Prp8 and Snu114 in vitro and in vivo.
Title: Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2.
Results report the structure of the Saccharomyces cerevisiae tri-snRNP, and show that U4/U6 snRNP forms a domain termed the arm, while a separate head domain adjacent to the arm harbors Brr2, and Prp8 and the GTPase Snu114 are located centrally.
Title: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)	IBA Inferred from Biological aspect of Ancestor more info
involved_in spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of U4/U6 x U5 tri-snRNP complex	IDA Inferred from Direct Assay more info	PubMed
part_of U5 snRNP	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IBA Inferred from Biological aspect of Ancestor more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-dependent RNA helicase BRR2

NP_011099.1

EC 3.6.4.13

RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

529530..536021 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179062.1 → NP_011099.1 TPA: ATP-dependent RNA helicase BRR2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011099.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM80, P32639

UniProtKB/TrEMBL

A0A8H8UM35, G2WD58, N1P3P9

Conserved Domains (4) summary

smart00611
Location:1843 → 2161

SEC63; Domain of unknown function in Sec63p, Brr2p and other proteins

COG1204
Location:462 → 1263

BRR2; Replicative superfamily II helicase [Replication, recombination and repair]

cd18021
Location:1345 → 1536

DEXHc_Brr2_2; C-terminal D[D/E]X[H/Q]-box helicase domain of spliceosomal Brr2 RNA helicase

pfam18149
Location:281 → 389

Helicase_PWI; N-terminal helicase PWI domain