ATG8 ubiquitin-like protein ATG8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ATG8
Gene description: ubiquitin-like protein ATG8
Primary source: SGD:S000000174
Locus tag: YBL078C
See related: AllianceGenome:SGD:S000000174
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: APG8; AUT7; CVT5
Summary: Enables phosphatidylethanolamine binding activity and protein tag. Involved in several processes, including autophagy of nucleus; macroautophagy; and protein targeting to vacuole. Located in several cellular components, including lipid droplet; vacuole; and vacuole-isolation membrane contact site. Is extrinsic component of membrane. Human ortholog(s) of this gene implicated in glioblastoma. Orthologous to several human genes including GABARAPL1 (GABA type A receptor associated protein like 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (80378..80731, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Effect of ATG8 or SAC1 deficiency on the cell proliferation and lifespan of the long-lived PMT1 deficiency yeast cells.
Title: Effect of ATG8 or SAC1 deficiency on the cell proliferation and lifespan of the long-lived PMT1 deficiency yeast cells.
Biophysical characterization of the interaction of Atg8 with a disordered region of Nup159 involved in selective autophagy of the nuclear pore complex.
Title: Biophysical characterization of the interaction of Atg8 with a disordered region of Nup159 involved in selective autophagy of the nuclear pore complex.
Small but mighty: Atg8s and Rabs in membrane dynamics during autophagy.
Title: Small but mighty: Atg8s and Rabs in membrane dynamics during autophagy.
Membrane perturbation by lipidated Atg8 underlies autophagosome biogenesis.
Title: Membrane perturbation by lipidated Atg8 underlies autophagosome biogenesis.
TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes.
Title: TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes.
Atg3 promotes Atg8 lipidation via altering lipid diffusion and rearrangement.
Title: Atg3 promotes Atg8 lipidation via altering lipid diffusion and rearrangement.
Role of Atg8 in the regulation of vacuolar membrane invagination.
Title: Role of Atg8 in the regulation of vacuolar membrane invagination.
Septins also colocalized with autophagosomes, where they physically interacted with the autophagy proteins Atg8 and Atg9. When autophagosome degradation was blocked in septin-mutant cells, fewer autophagic structures accumulated, and an autophagy mutant defective in early stages of autophagosome biogenesis (atg1Delta), displayed decreased septin localization to the pre-autophagosomal structure.
Title: Septins are involved at the early stages of macroautophagy in S. cerevisiae.
The authors find that Mon1-Ccz1 directly binds to Atg8, the yeast homolog of the members of the mammalian LC3 protein family.
Title: Molecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structure.
The purified autophagy receptor ATG19 is recruited to affinity resins via a suitable tag and then added to fluorescently labeled ATG8 in solution. The relative strength of the interaction is assessed by determination of the fluorescence intensity on the surface of the bead at an equilibrium binding state. The relative binding strength is determined.
Title: Studies of Receptor-Atg8 Interactions During Selective Autophagy.

Submit: New GeneRIF Correction See all GeneRIFs (38)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylethanolamine binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylethanolamine binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein tag activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagosome maturation	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in autophagy of mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy of mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to nitrogen starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IPI Inferred from Physical Interaction more info	PubMed
involved_in lipid droplet formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in membrane fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to phagophore assembly site	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to vacuole involved in autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein-containing complex localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of membrane invagination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reticulophagy	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Cvt vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in autophagosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in autophagosome membrane	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IPI Inferred from Physical Interaction more info	PubMed
is_active_in lipid droplet	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in phagophore assembly site	IDA Inferred from Direct Assay more info	PubMed
located_in vacuole	IEA Inferred from Electronic Annotation more info
located_in vacuole-isolation membrane contact site	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ubiquitin-like protein ATG8

NP_009475.1

Ubiquitin-like protein conjugated to phosphatidylethanolamine (PE); role in membrane fusion and phagophore expansion during autophagosome formation; deconjugation is also required for biogenesis; targets Atg1p to autophagosomes; binds to the Atg1p-Atg13p complex, triggers its vacuolar degradation; role in vacuolar membrane protein turnover during early stationary phase; component of autophagosomes and Cvt vesicles; targeted to vacuole via AP-3 pathway

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

80378..80731 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178318.1 → NP_009475.1 TPA: ubiquitin-like protein ATG8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009475.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZKM4, D6VPS5, P38182

UniProtKB/TrEMBL

A0A6A5PZP1, B3LNL0, C7GK56, C8Z3R6, G2W8T4, N1P6W4

Conserved Domains (1) summary

cd16128
Location:11 → 113

Ubl_ATG8; ubiquitin-like (Ubl) domain found in Saccharomyces cerevisiae Atg8p and related proteins; sub-family of the autophagy-related 8 (ATG8) family