Brsk2 BR serine/threonine kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Brsk2provided by MGI
Official Full Name: BR serine/threonine kinase 2provided by MGI
Primary source: MGI:MGI:1923020
See related: Ensembl:ENSMUSG00000053046 AllianceGenome:MGI:1923020
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SADA; SAD-A; 4833424K13Rik
Summary: Enables protein kinase binding activity; protein serine/threonine kinase activity; and tau-protein kinase activity. Involved in several processes, including microtubule cytoskeleton organization involved in establishment of planar polarity; regulation of axonogenesis; and regulation of insulin secretion involved in cellular response to glucose stimulus. Acts upstream of or within neuron projection morphogenesis and peptidyl-serine phosphorylation. Predicted to be located in distal axon and endoplasmic reticulum. Predicted to be active in centrosome and cytoplasm. Is expressed in several structures, including central nervous system and retina. Orthologous to human BRSK2 (BR serine/threonine kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in whole brain E14.5 (RPKM 31.8), CNS E14 (RPKM 28.8) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F5; 7 87.43 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (141503488..141557981)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (141949751..142004244)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

BRSK2 in pancreatic beta cells promotes hyperinsulinemia-coupled insulin resistance and its genetic variants are associated with human type 2 diabetes.
Title: BRSK2 in pancreatic β cells promotes hyperinsulinemia-coupled insulin resistance and its genetic variants are associated with human type 2 diabetes.
Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex.
Title: Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex.
SAD-A is activated by the incretin hormone GLP-1 in pancreatic beta-cell. SAD-A promotes the phosphorylation of Bad S155.
Title: SAD-A, a downstream mediator of GLP-1 signaling, promotes the phosphorylation of Bad S155 to regulate in vitro β-cell functions.
Glucose and incretin stimulate insulin secretion through the phosphorylation of GDIalpha at Ser174 by SAD-A, which leads to the activation of Rho GTPases, culminating in insulin exocytosis.
Title: SAD-A Promotes Glucose-Stimulated Insulin Secretion Through Phosphorylation and Inhibition of GDIα in Male Islet β Cells.
Taken together, these data suggest a model for synergistic autoinhibition and membrane activation of SAD kinases.
Title: Structural insight into the mechanism of synergistic autoinhibition of SAD kinases.
SAD-A and AMPK kinases are regulators of mTORC1 signaling in the pancreatic beta-cells.
Title: SAD-A and AMPK kinases: the "yin and yang" regulators of mTORC1 signaling in pancreatic β cells.
SAD-A and SAD-B kinases have multiple, sequential roles in axonal differentiation
Title: SAD kinases control the maturation of nerve terminals in the mammalian peripheral and central nervous systems.
SAD-A kinase controls islet beta-cell size and function as a mediator of mTORC1 signaling.
Title: SAD-A kinase controls islet β-cell size and function as a mediator of mTORC1 signaling.
SAD-A kinase is a pancreas-specific mediator of incretin response in islet beta cells.
Title: SAD-A potentiates glucose-stimulated insulin secretion as a mediator of glucagon-like peptide 1 response in pancreatic β cells.
a novel function of BRSK2 in the regulation of GSIS in beta-cells via a PCTAIRE1-dependent mechanism and suggest that BRSK2 is an attractive target for developing novel diabetic drugs.
Title: Brain-selective kinase 2 (BRSK2) phosphorylation on PCTAIRE1 negatively regulates glucose-stimulated insulin secretion in pancreatic β-cells.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH131512 (e-PCR)
- UniSTS:214795

RH143841 (e-PCR)
- UniSTS:233087

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATPase binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IC Inferred by Curator more info	PubMed
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables tau-protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables tau-protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables tau-protein kinase activity	ISO Inferred from Sequence Orthology more info
enables tau-protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ERAD pathway	ISO Inferred from Sequence Orthology more info
involved_in G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in establishment of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within establishment of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within exocytosis	IEA Inferred from Electronic Annotation more info
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in microtubule cytoskeleton organization involved in establishment of planar polarity	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neuron projection morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of insulin secretion involved in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of insulin secretion involved in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuron projection development	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in distal axon	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase BRSK2

Names: BR serine/threonine-protein kinase 2; brain-specific serine/threonine-protein kinase 2; serine/threonine-protein kinase SAD-A

NP_001009929.1

EC 2.7.11.1

EC 2.7.11.26

NP_001009930.1

EC 2.7.11.1

EC 2.7.11.26

NP_001263692.1

EC 2.7.11.1

EC 2.7.11.26

NP_083702.1

EC 2.7.11.1

EC 2.7.11.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001009929.3 → NP_001009929.1 serine/threonine-protein kinase BRSK2 isoform beta

See identical proteins and their annotated locations for NP_001009929.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks a 3' coding exon and contains an additional 3' UTR exon and uses an alternate splice junction at the 5' end of the last exon compared to isoform 3. The resulting isoform (beta) has a shorter and distinct C-terminus compared to isoform gamma.

Source sequence(s)

AK158574, AL603836, AL772165

Consensus CDS

CCDS22023.1

UniProtKB/TrEMBL

G3UXM5

Related

ENSMUSP00000077330.6, ENSMUST00000078200.12

Conserved Domains (2) summary

cd14340
Location:298 → 351

UBA_BRSK; UBA domain found in serine/threonine-protein kinase BRSK1, BRSK2 and similar proteins

cd14081
Location:18 → 271

STKc_BRSK1_2; Catalytic domain of Brain-specific serine/threonine-protein kinases 1 and 2
NM_001009930.3 → NP_001009930.1 serine/threonine-protein kinase BRSK2 isoform gamma

See identical proteins and their annotated locations for NP_001009930.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (gamma).

Source sequence(s)

AK158574, AL603836, AL772165, BE986320

Consensus CDS

CCDS22022.1

UniProtKB/TrEMBL

G3UXM5

Related

ENSMUSP00000074969.6, ENSMUST00000075528.12

Conserved Domains (2) summary

cd14340
Location:298 → 351

UBA_BRSK; UBA domain found in serine/threonine-protein kinase BRSK1, BRSK2 and similar proteins

cd14081
Location:18 → 271

STKc_BRSK1_2; Catalytic domain of Brain-specific serine/threonine-protein kinases 1 and 2
NM_001276763.1 → NP_001263692.1 serine/threonine-protein kinase BRSK2 isoform delta

See identical proteins and their annotated locations for NP_001263692.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) has multiple differences in the coding region and 3' UTR, compared to variant 3. The resulting protein (isoform delta) is shorter than isoform gamma.

Source sequence(s)

AL603836, AL772165

UniProtKB/TrEMBL

G3FEZ6, G3UXM5

Conserved Domains (2) summary

cd14340
Location:298 → 351

UBA_BRSK; UBA domain found in serine/threonine-protein kinase BRSK1, BRSK2 and similar proteins

cd14081
Location:18 → 271

STKc_BRSK1_2; Catalytic domain of Brain-specific serine/threonine-protein kinases 1 and 2
NM_029426.2 → NP_083702.1 serine/threonine-protein kinase BRSK2 isoform alpha

See identical proteins and their annotated locations for NP_083702.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) lacks an alternate in-frame 3' exon and uses an alternate splice junction at the 5' end of the last exon compared to variant 3. The resulting isoform (alpha) has a shorter and distinct C-terminus compared to isoform gamma.

Source sequence(s)

AL603836, AL772165

Consensus CDS

CCDS22021.1

UniProtKB/TrEMBL

G3UXM5

Related

ENSMUSP00000018971.9, ENSMUST00000018971.15

Conserved Domains (2) summary

cd14340
Location:298 → 351

UBA_BRSK; UBA domain found in serine/threonine-protein kinase BRSK1, BRSK2 and similar proteins

cd14081
Location:18 → 271

STKc_BRSK1_2; Catalytic domain of Brain-specific serine/threonine-protein kinases 1 and 2