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PRKDC protein kinase, DNA-activated, catalytic polypeptide [ Homo sapiens (human) ]

Gene ID: 5591, updated on 19-Jul-2014
Official Symbol
PRKDCprovided by HGNC
Official Full Name
protein kinase, DNA-activated, catalytic polypeptideprovided by HGNC
Primary source
HGNC:9413
See related
Ensembl:ENSG00000253729; HPRD:02941; MIM:600899
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs
Summary
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
See PRKDC in Epigenomics, MapViewer
Location:
8q11
Exon count :
86
Annotation release Status Assembly Chr Location
106 current GRCh38 (GCF_000001405.26) 8 NC_000008.11 (47773108..47960183, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 8 NC_000008.10 (48685669..48872743, complement)

Chromosome 8 - NC_000008.11Genomic Context describing neighboring genes Neighboring gene scaffolding protein involved in DNA repair Neighboring gene ubiquitin-conjugating enzyme E2N pseudogene Neighboring gene CCAAT/enhancer binding protein (C/EBP), delta Neighboring gene minichromosome maintenance complex component 4 Neighboring gene ubiquitin-conjugating enzyme E2 variant 2

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify catalytic polypeptide of DNA-activated protein kinase (PRKDC), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify catalytic polypeptide of DNA-activated protein kinase (PRKDC), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify catalytic polypeptide of DNA-activated protein kinase (PRKDC), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify catalytic polypeptide of DNA-activated protein kinase (PRKDC), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Rev rev HIV-1 Rev interacting protein, DNA-dependent protein kinase catalytic subunit (PRKDC), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with PRKDC is increased by RRE PubMed
Tat tat HIV-1 Tat is phosphorylated by DNA-PK at serine positions 16 and 62 and DNA-PK-induced Tat phosphorylation contributes significantly to HIV-1 replication PubMed
tat The DNA repair gene DNA-PKcs and cell cycle-related genes Cdc20, Cdc25C, KIF2C and CTS1 are downregulated in HIV-1 Tat-expressing human rhabdomyosarcoma cells PubMed
tat Amino acids 56-101 of HIV-1 Tat mediate Tat binding to DNA-PK, an effect that augments DNA-PK-mediated phosphorylation of Sp1 during Tat transactivation of the HIV-1 LTR promoter PubMed
Vpu vpu HIV-1 Vpu is identified to have a physical interaction with protein kinase, DNA-activated, catalytic polypeptide (PRKDC) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
integrase gag-pol HIV-1 IN-mediated proviral DNA integration triggers cell death during HIV-1 infection. The mechanism of killing during viral integration involves activation of DNA-PK, which causes phosphorylation of p53 and histone gammaH2AX PubMed
gag-pol DNA-PK is proposed to play a role in retroviral DNA integration and protects cells against toxicity induced by HIV-1 Integrase or integration PubMed

Go to the HIV-1, Human Protein Interaction Database

  • BARD1 signaling events, organism-specific biosystem (from Pathway Interaction Database)
    BARD1 signaling events, organism-specific biosystem
    BARD1 signaling events
  • Cell cycle, organism-specific biosystem (from WikiPathways)
    Cell cycle, organism-specific biosystemThe cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle involves processes crucial to the survival of a c...
  • Cell cycle, organism-specific biosystem (from KEGG)
    Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • Cell cycle, conserved biosystem (from KEGG)
    Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • Class I PI3K signaling events mediated by Akt, organism-specific biosystem (from Pathway Interaction Database)
    Class I PI3K signaling events mediated by Akt, organism-specific biosystem
    Class I PI3K signaling events mediated by Akt
  • Coregulation of Androgen receptor activity, organism-specific biosystem (from Pathway Interaction Database)
    Coregulation of Androgen receptor activity, organism-specific biosystem
    Coregulation of Androgen receptor activity
  • Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem (from REACTOME)
    Cytosolic sensors of pathogen-associated DNA, organism-specific biosystemPresence of pathogen-associated DNA in cytosol induces type I IFN production. Several intracellular receptors have been implicated to some degree. These include DNA-dependent activator of interferon ...
  • DNA Repair, organism-specific biosystem (from REACTOME)
    DNA Repair, organism-specific biosystemDNA repair is a phenomenal multi-enzyme, multi-pathway system required to ensure the integrity of the cellular genome. These cellular mechanisms that must cope with the plethora of DNA base pair ad...
  • DNA-PK complex, organism-specific biosystem (from KEGG)
    DNA-PK complex, organism-specific biosystemStructural complex; Genetic information processing; Repair system
  • DNA-PK complex, conserved biosystem (from KEGG)
    DNA-PK complex, conserved biosystemStructural complex; Genetic information processing; Repair system
  • Double-Strand Break Repair, organism-specific biosystem (from REACTOME)
    Double-Strand Break Repair, organism-specific biosystemNumerous types of DNA damage can occur within a cell due to the endogenous production of oxygen free radicals, normal alkylation reactions, or exposure to exogenous radiations and chemicals. Double-s...
  • FAS pathway and Stress induction of HSP regulation, organism-specific biosystem (from WikiPathways)
    FAS pathway and Stress induction of HSP regulation, organism-specific biosystemThis pathway describes the Fas induced apoptosis and interplay with Hsp27 in response to stress. More info: [http://www.biocarta.com/pathfiles/h_hsp27Pathway.asp BioCarta].
  • IRF3-mediated induction of type I IFN, organism-specific biosystem (from REACTOME)
    IRF3-mediated induction of type I IFN, organism-specific biosystemTANK-binding kinase 1 (TBK1) and interferon regulatory factor 3 (IRF3) are central regulators of type-I interferon induction during bacterial or viral infection. TBK1 was found to form complexes with...
  • Immune System, organism-specific biosystem (from REACTOME)
    Immune System, organism-specific biosystemHumans are exposed to millions of potential pathogens daily, through contact, ingestion, and inhalation. Our ability to avoid infection depends on the adaptive immune system and during the first crit...
  • Innate Immune System, organism-specific biosystem (from REACTOME)
    Innate Immune System, organism-specific biosystemInnate immunity encompases the nonspecific part of immunity tha are part of an individual's natural biologic makeup
  • Non-homologous end-joining, organism-specific biosystem (from KEGG)
    Non-homologous end-joining, organism-specific biosystemNonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX c...
  • Non-homologous end-joining, conserved biosystem (from KEGG)
    Non-homologous end-joining, conserved biosystemNonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX c...
  • Nonhomologous End-joining (NHEJ), organism-specific biosystem (from REACTOME)
    Nonhomologous End-joining (NHEJ), organism-specific biosystemThe NHEJ pathway is initiated in response to the formation of a DNA double-strand break (DSB) induced by a DNA-damaging agent such as ionizing radiation. First, the Ku70/80 heterodimer binds to the ...
  • Processing of DNA ends prior to end rejoining, organism-specific biosystem (from REACTOME)
    Processing of DNA ends prior to end rejoining, organism-specific biosystemIonizing radiation induced DNA double strand breaks often contain end groups that must be processed before the DNA can be rejoined. Possible candidate proteins for the processing steps include Artem...
  • Prostate Cancer, organism-specific biosystem (from WikiPathways)
    Prostate Cancer, organism-specific biosystem
    Prostate Cancer
  • RB in Cancer, organism-specific biosystem (from WikiPathways)
    RB in Cancer, organism-specific biosystem
    RB in Cancer
  • STING mediated induction of host immune responses, organism-specific biosystem (from REACTOME)
    STING mediated induction of host immune responses, organism-specific biosystemSTING (stimulator of IFN genes; also known as MITA/ERIS/MPYS/TMEM173) is an endoplasmic reticulum (ER) resident, which is required for effective type I IFN production in response to nucleic acids. In...
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
DNA binding IEA
Inferred from Electronic Annotation
more info
 
DNA-dependent protein kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IEA
Inferred from Electronic Annotation
more info
 
poly(A) RNA binding IDA
Inferred from Direct Assay
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase activity TAS
Traceable Author Statement
more info
PubMed 
protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
transcription factor binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
B cell lineage commitment IEA
Inferred from Electronic Annotation
more info
 
DNA repair TAS
Traceable Author Statement
more info
 
T cell differentiation in thymus IEA
Inferred from Electronic Annotation
more info
 
T cell lineage commitment IEA
Inferred from Electronic Annotation
more info
 
T cell receptor V(D)J recombination IEA
Inferred from Electronic Annotation
more info
 
brain development IEA
Inferred from Electronic Annotation
more info
 
cellular protein modification process TAS
Traceable Author Statement
more info
PubMed 
cellular response to insulin stimulus IMP
Inferred from Mutant Phenotype
more info
PubMed 
double-strand break repair TAS
Traceable Author Statement
more info
 
double-strand break repair via nonhomologous end joining TAS
Traceable Author Statement
more info
 
ectopic germ cell programmed cell death IEA
Inferred from Electronic Annotation
more info
 
heart development IEA
Inferred from Electronic Annotation
more info
 
immunoglobulin V(D)J recombination IEA
Inferred from Electronic Annotation
more info
 
innate immune response TAS
Traceable Author Statement
more info
 
intrinsic apoptotic signaling pathway in response to DNA damage IEA
Inferred from Electronic Annotation
more info
 
negative regulation of protein phosphorylation ISS
Inferred from Sequence or Structural Similarity
more info
 
peptidyl-serine phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of apoptotic process IEA
Inferred from Electronic Annotation
more info
 
positive regulation of transcription from RNA polymerase II promoter IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of type I interferon production TAS
Traceable Author Statement
more info
 
pro-B cell differentiation IEA
Inferred from Electronic Annotation
more info
 
protein destabilization IEA
Inferred from Electronic Annotation
more info
 
regulation of circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
 
response to gamma radiation IEA
Inferred from Electronic Annotation
more info
 
rhythmic process IEA
Inferred from Electronic Annotation
more info
 
signal transduction involved in mitotic G1 DNA damage checkpoint IMP
Inferred from Mutant Phenotype
more info
 
somitogenesis IEA
Inferred from Electronic Annotation
more info
 
telomere maintenance IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
DNA-dependent protein kinase-DNA ligase 4 complex IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
membrane IDA
Inferred from Direct Assay
more info
PubMed 
nonhomologous end joining complex IDA
Inferred from Direct Assay
more info
 
nucleolus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
 
transcription factor complex IDA
Inferred from Direct Assay
more info
PubMed 
Preferred Names
DNA-dependent protein kinase catalytic subunit
Names
DNA-dependent protein kinase catalytic subunit
p460
DNA-PK catalytic subunit
hyper-radiosensitivity of murine scid mutation, complementing 1
NP_001075109.1
NP_008835.5

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_023435.1 

    Range
    5001..192076
    Download
    GenBank, FASTA, Sequence Viewer (Graphics), LRG_162

mRNA and Protein(s)

  1. NM_001081640.1NP_001075109.1  DNA-dependent protein kinase catalytic subunit isoform 2

    See proteins identical to NP_001075109.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    U34994, U47077
    UniProtKB/Swiss-Prot
    P78527
    Related
    ENSP00000345182, ENST00000338368
    Conserved Domains (4) summary
    cd05172
    Location:37193984
    Blast Score: 1068
    PIKKc_DNA-PK; DNA-dependent protein kinase (DNA-PK), catalytic domain; The DNA-PK catalytic domain subfamily is part of a larger superfamily that includes the catalytic domains of other kinases such as the typical serine/threonine/tyrosine protein kinases (PKs), ...
    pfam02259
    Location:30233470
    Blast Score: 584
    FAT; FAT domain
    pfam02260
    Location:40664097
    Blast Score: 118
    FATC; FATC domain
    pfam08163
    Location:18152210
    Blast Score: 1645
    NUC194; NUC194 domain
  2. NM_006904.6NP_008835.5  DNA-dependent protein kinase catalytic subunit isoform 1

    See proteins identical to NP_008835.5

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    U47077
    UniProtKB/Swiss-Prot
    P78527
    Related
    ENSP00000313420, ENST00000314191
    Conserved Domains (4) summary
    cd05172
    Location:37194015
    Blast Score: 1127
    PIKKc_DNA-PK; DNA-dependent protein kinase (DNA-PK), catalytic domain; The DNA-PK catalytic domain subfamily is part of a larger superfamily that includes the catalytic domains of other kinases such as the typical serine/threonine/tyrosine protein kinases (PKs), ...
    pfam02259
    Location:30233470
    Blast Score: 583
    FAT; FAT domain
    pfam02260
    Location:40974128
    Blast Score: 118
    FATC; FATC domain
    pfam08163
    Location:18152210
    Blast Score: 1644
    NUC194; NUC194 domain

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38 Primary Assembly

Genomic

  1. NC_000008.11 

    Range
    47773108..47960183
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

  1. AC_000140.1 

    Range
    44148581..44335706
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.1

Genomic

  1. NC_018919.2 

    Range
    48738053..48925105
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

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