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PKM pyruvate kinase, muscle [ Homo sapiens (human) ]

Gene ID: 5315, updated on 11-Sep-2014
Official Symbol
PKMprovided by HGNC
Official Full Name
pyruvate kinase, muscleprovided by HGNC
Primary source
HGNC:HGNC:9021
See related
Ensembl:ENSG00000067225; HPRD:01529; MIM:179050; Vega:OTTHUMG00000172709
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Summary
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Location:
15q22
Exon count:
16
Annotation release Status Assembly Chr Location
106 current GRCh38 (GCF_000001405.26) 15 NC_000015.10 (72199029..72231818, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 15 NC_000015.9 (72491370..72523901, complement)

Chromosome 15 - NC_000015.10Genomic Context describing neighboring genes Neighboring gene SUMO/sentrin specific peptidase family member 8 Neighboring gene ribosomal protein L21 pseudogene 115 Neighboring gene GRAM domain containing 2 Neighboring gene uncharacterized LOC102723606 Neighboring gene poly (ADP-ribose) polymerase family, member 6 Neighboring gene CUGBP, Elav-like family member 6

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 is identified to have a physical interaction with pyruvate kinase, muscle (PKM2) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Tat tat Expression of HIV-1 Tat upregulates the abundance of pyruvate kinase, muscle (PKM) in the nucleoli of Jurkat T-cells PubMed
Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of pyruvate kinase, muscle (PKM) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human pyruvate kinase, muscle (PKM) at amino acid residues 306-307 and 359-361 by the HIV-1 protease PubMed

Go to the HIV-1, Human Protein Interaction Database

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Clone Names

  • MGC3932

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
MHC class II protein complex binding IDA
Inferred from Direct Assay
more info
 
magnesium ion binding IEA
Inferred from Electronic Annotation
more info
 
poly(A) RNA binding IDA
Inferred from Direct Assay
more info
 
potassium ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
pyruvate kinase activity TAS
Traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
carbohydrate metabolic process TAS
Traceable Author Statement
more info
 
glucose metabolic process TAS
Traceable Author Statement
more info
 
glycolytic process IEA
Inferred from Electronic Annotation
more info
 
glycolytic process TAS
Traceable Author Statement
more info
 
phosphorylation IEA
Inferred from Electronic Annotation
more info
 
programmed cell death IDA
Inferred from Direct Assay
more info
PubMed 
small molecule metabolic process TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
cilium IEA
Inferred from Electronic Annotation
more info
 
cytoplasm IDA
Inferred from Direct Assay
more info
 
cytosol NAS
Non-traceable Author Statement
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
extracellular matrix IDA
Inferred from Direct Assay
more info
 
extracellular vesicular exosome IDA
Inferred from Direct Assay
more info
PubMed 
mitochondrion IDA
Inferred from Direct Assay
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
plasma membrane IDA
Inferred from Direct Assay
more info
 
vesicle IDA
Inferred from Direct Assay
more info
PubMed 
Preferred Names
pyruvate kinase PKM
Names
pyruvate kinase PKM
p58
OIP-3
tumor M2-PK
PK, muscle type
pyruvate kinase 2/3
OPA-interacting protein 3
pyruvate kinase isozymes M1/M2
pyruvate kinase muscle isozyme
thyroid hormone-binding protein 1
epididymis secretory protein Li 30
cytosolic thyroid hormone-binding protein
thyroid hormone-binding protein, cytosolic
NP_001193725.1
NP_001193726.1
NP_001193727.1
NP_001193728.1
NP_002645.3
NP_872270.1
NP_872271.1

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001206796.1NP_001193725.1  pyruvate kinase PKM isoform c

    See proteins identical to NP_001193725.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (c).
    Source sequence(s)
    AK294315, BC000481, BC035198, DA382029, DC376054
    UniProtKB/Swiss-Prot
    P14618
    Conserved Domains (2) summary
    TIGR01064
    Location:117601
    Blast Score: 1980
    pyruv_kin; pyruvate kinase
    cd00288
    Location:116604
    Blast Score: 2159
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  2. NM_001206797.1NP_001193726.1  pyruvate kinase PKM isoform d

    See proteins identical to NP_001193726.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence, lacks an alternate in-frame segment, and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (d) is shorter at the N-terminus, lacks an alternate internal segment, and has a different internal segment compared to isoform c.
    Source sequence(s)
    AK297951, BC000481, BC035198, DC376054
    Consensus CDS
    CCDS73752.1
    UniProtKB/TrEMBL
    B4DNK4
    UniProtKB/Swiss-Prot
    P14618
    Related
    ENSP00000373745, ENST00000389093
    Conserved Domains (2) summary
    TIGR01064
    Location:43453
    Blast Score: 1601
    pyruv_kin; pyruvate kinase
    cd00288
    Location:42456
    Blast Score: 1791
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  3. NM_001206798.1NP_001193727.1  pyruvate kinase PKM isoform e

    See proteins identical to NP_001193727.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, lacks an alternate exon, and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (e) has a shorter and distinct N-terminus, lacks an alternate internal segment, and has a different internal segment compared to isoform c.
    Source sequence(s)
    AK300800, BC000481, BC035198, DC376054
    Consensus CDS
    CCDS55972.1
    UniProtKB/Swiss-Prot
    P14618
    Related
    ENSP00000403365, ENST00000449901
    Conserved Domains (2) summary
    TIGR01064
    Location:68512
    Blast Score: 1762
    pyruv_kin; pyruvate kinase
    cd00288
    Location:68515
    Blast Score: 1955
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  4. NM_001206799.1NP_001193728.1  pyruvate kinase PKM isoform f

    See proteins identical to NP_001193728.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (7) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (f) has a shorter and distinct N-terminus compared to isoform c.
    Source sequence(s)
    AK294315, AK309781, BC000481, BC035198
    UniProtKB/Swiss-Prot
    P14618
    Conserved Domains (2) summary
    TIGR01064
    Location:48532
    Blast Score: 1955
    pyruv_kin; pyruvate kinase
    cd00288
    Location:47535
    Blast Score: 2139
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  5. NM_002654.4NP_002645.3  pyruvate kinase PKM isoform a

    See proteins identical to NP_002645.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (a, also called M2) is shorter at the N-terminus and has a different internal segment compared to isoform c.
    Source sequence(s)
    BC000481, BC035198, DC376054
    Consensus CDS
    CCDS32284.1
    UniProtKB/Swiss-Prot
    P14618
    UniProtKB/TrEMBL
    V9HWB8
    Related
    ENSP00000334983, OTTHUMP00000249141, ENST00000335181, OTTHUMT00000420056
    Conserved Domains (2) summary
    TIGR01064
    Location:43527
    Blast Score: 1942
    pyruv_kin; pyruvate kinase
    cd00288
    Location:42530
    Blast Score: 2142
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  6. NM_182470.2NP_872270.1  pyruvate kinase PKM isoform b

    See proteins identical to NP_872270.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c.
    Source sequence(s)
    AK092369, BC023592, BC035198, DC376054
    Consensus CDS
    CCDS32285.1
    UniProtKB/TrEMBL
    A0A024R5Z9
    UniProtKB/Swiss-Prot
    P14618
    Related
    ENSP00000320171, ENST00000319622
    Conserved Domains (2) summary
    TIGR01064
    Location:43527
    Blast Score: 1956
    pyruv_kin; pyruvate kinase
    cd00288
    Location:42530
    Blast Score: 2140
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  7. NM_182471.2NP_872271.1  pyruvate kinase PKM isoform b

    See proteins identical to NP_872271.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c.
    Source sequence(s)
    BC023592, BC035198, DC376054
    Consensus CDS
    CCDS32285.1
    UniProtKB/TrEMBL
    A0A024R5Z9
    UniProtKB/Swiss-Prot
    P14618
    Conserved Domains (2) summary
    TIGR01064
    Location:43527
    Blast Score: 1956
    pyruv_kin; pyruvate kinase
    cd00288
    Location:42530
    Blast Score: 2140
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38 Primary Assembly

Genomic

  1. NC_000015.10 

    Range
    72199029..72231818
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005254445.2XP_005254502.1  

    See proteins identical to XP_005254502.1

    UniProtKB/Swiss-Prot
    P14618
    UniProtKB/TrEMBL
    V9HWB8
    Conserved Domains (2) summary
    TIGR01064
    Location:43527
    Blast Score: 1942
    pyruv_kin; pyruvate kinase
    cd00288
    Location:42530
    Blast Score: 2142
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  2. XM_006720570.1XP_006720633.1  

    Conserved Domains (2) summary
    TIGR01064
    Location:117601
    Blast Score: 1966
    pyruv_kin; pyruvate kinase
    cd00288
    Location:116604
    Blast Score: 2159
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
  3. XM_005254443.1XP_005254500.1  

    Conserved Domains (2) summary
    TIGR01064
    Location:103587
    Blast Score: 1959
    pyruv_kin; pyruvate kinase
    cd00288
    Location:102590
    Blast Score: 2157
    Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...

Alternate HuRef

Genomic

  1. AC_000147.1 

    Range
    49321965..49354312
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.1

Genomic

  1. NC_018926.2 

    Range
    72609505..72641870
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)