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TOP2A topoisomerase (DNA) II alpha 170kDa [ Homo sapiens (human) ]

Gene ID: 7153, updated on 15-Sep-2014
Official Symbol
TOP2Aprovided by HGNC
Official Full Name
topoisomerase (DNA) II alpha 170kDaprovided by HGNC
Primary source
HGNC:HGNC:11989
See related
Ensembl:ENSG00000131747; HPRD:00536; MIM:126430; Vega:OTTHUMG00000155008
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TOP2; TP2A
Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
See TOP2A in Epigenomics, MapViewer
Location:
17q21-q22
Exon count:
36
Annotation release Status Assembly Chr Location
106 current GRCh38 (GCF_000001405.26) 17 NC_000017.11 (40388521..40417950, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (38544773..38574202, complement)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene RNA, 5S ribosomal pseudogene 441 Neighboring gene kelch repeat and BTB (POZ) domain containing 2 pseudogene Neighboring gene ribosomal protein L23a pseudogene 75 Neighboring gene insulin-like growth factor binding protein 4 Neighboring gene uncharacterized LOC102724129

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Replication interactions

Interaction Pubs
Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases HIV-1 replication in Sup T1 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 is identified to have a physical interaction with topoisomerase (DNA) II alpha 170kDa (TOP2A) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Tat tat Interaction of HIV-1 Tat with TOP2A in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry PubMed
capsid gag Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases p24 levels in HIV-1 infected cell culture supernatants when compared to control PubMed
reverse transcriptase gag-pol HIV-1 RT co-localizes with TOP2A and TOP2B in cytosol of HIV-1 infected Sup T1 and PBMC cells PubMed

Go to the HIV-1, Human Interaction Database

  • Cell Cycle, organism-specific biosystem (from REACTOME)
    Cell Cycle, organism-specific biosystem
    Cell Cycle
  • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
    Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
  • G0 and Early G1, organism-specific biosystem (from REACTOME)
    G0 and Early G1, organism-specific biosystemIn G0 and early G1 in quiescent cells, p130 (RBL2) bound to E2F4 or E2F5 and either DP1 or DP2, associates with the MuvB complex, forming an evolutionarily conserved DREAM complex, that represses tra...
  • Gastric cancer network 1, organism-specific biosystem (from WikiPathways)
    Gastric cancer network 1, organism-specific biosystemNetwork generated by mapping candidate oncogenes and tumor suppressor genes identified by integrated analysis of expression array and aCGH data. Networks generated by Ingenuity Pathway Analysis.
  • Gastric cancer network 2, organism-specific biosystem (from WikiPathways)
    Gastric cancer network 2, organism-specific biosystemNetwork generated by mapping candidate oncogenes and tumor suppressor genes identified by integrated analysis of expression array and aCGH data. Network generated by Ingenuity Pathway Analysis.
  • Integrated Pancreatic Cancer Pathway, organism-specific biosystem (from WikiPathways)
    Integrated Pancreatic Cancer Pathway, organism-specific biosystemAn integrated pathway model which displays the protein-protein interactions (PPIs) among the relevant proteins for pancreatic cancer. This pathway is a collection of different mechanistic protein pat...
  • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
    Mitotic G1-G1/S phases, organism-specific biosystem
    Mitotic G1-G1/S phases
  • RB in Cancer, organism-specific biosystem (from WikiPathways)
    RB in Cancer, organism-specific biosystem
    RB in Cancer
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
DNA binding, bending IDA
Inferred from Direct Assay
more info
 
DNA topoisomerase type II (ATP-hydrolyzing) activity IDA
Inferred from Direct Assay
more info
PubMed 
DNA-dependent ATPase activity IDA
Inferred from Direct Assay
more info
PubMed 
chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
drug binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
histone deacetylase binding IPI
Inferred from Physical Interaction
more info
PubMed 
magnesium ion binding IDA
Inferred from Direct Assay
more info
 
poly(A) RNA binding IDA
Inferred from Direct Assay
more info
 
protein C-terminus binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein heterodimerization activity IPI
Inferred from Physical Interaction
more info
PubMed 
protein homodimerization activity IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C binding IPI
Inferred from Physical Interaction
more info
PubMed 
ubiquitin binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
Process Evidence Code Pubs
ATP catabolic process IDA
Inferred from Direct Assay
more info
PubMed 
ATP catabolic process IEA
Inferred from Electronic Annotation
more info
 
DNA ligation IDA
Inferred from Direct Assay
more info
PubMed 
DNA topological change IDA
Inferred from Direct Assay
more info
 
DNA unwinding involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
 
apoptotic chromosome condensation IDA
Inferred from Direct Assay
more info
PubMed 
cellular response to DNA damage stimulus IDA
Inferred from Direct Assay
more info
PubMed 
chromosome segregation IMP
Inferred from Mutant Phenotype
more info
PubMed 
embryonic cleavage IEA
Inferred from Electronic Annotation
more info
 
hematopoietic progenitor cell differentiation IEA
Inferred from Electronic Annotation
more info
 
mitotic DNA integrity checkpoint IBA
Inferred from Biological aspect of Ancestor
more info
 
mitotic cell cycle TAS
Traceable Author Statement
more info
 
mitotic recombination IBA
Inferred from Biological aspect of Ancestor
more info
 
positive regulation of apoptotic process IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of transcription from RNA polymerase II promoter IEA
Inferred from Electronic Annotation
more info
 
positive regulation of viral genome replication IMP
Inferred from Mutant Phenotype
more info
PubMed 
resolution of meiotic recombination intermediates IBA
Inferred from Biological aspect of Ancestor
more info
 
sister chromatid segregation IBA
Inferred from Biological aspect of Ancestor
more info
 
Component Evidence Code Pubs
DNA topoisomerase complex (ATP-hydrolyzing) IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with centriole IDA
Inferred from Direct Assay
more info
PubMed 
condensed chromosome IEA
Inferred from Electronic Annotation
more info
 
colocalizes_with nuclear chromosome IDA
Inferred from Direct Assay
more info
PubMed 
NOT nucleoid IBA
Inferred from Biological aspect of Ancestor
more info
 
nucleolus IDA
Inferred from Direct Assay
more info
 
NOT nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
 
protein complex IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with viral integration complex NAS
Non-traceable Author Statement
more info
PubMed 
Preferred Names
DNA topoisomerase 2-alpha
Names
DNA topoisomerase 2-alpha
DNA gyrase
DNA topoisomerase II, 170 kD
DNA topoisomerase (ATP-hydrolyzing)
DNA topoisomerase II, alpha isozyme
NP_001058.2

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_027678.1 

    Range
    5001..34430
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001067.3NP_001058.2  DNA topoisomerase 2-alpha

    See proteins identical to NP_001058.2

    Status: REVIEWED

    Source sequence(s)
    AC080112, BC140791, DB069825
    Consensus CDS
    CCDS45672.1
    UniProtKB/Swiss-Prot
    P11388
    Related
    ENSP00000411532, OTTHUMP00000207108, ENST00000423485, OTTHUMT00000338035
    Conserved Domains (6) summary
    cd03365
    Location:455575
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00075
    Location:81182
    HATPase_c; Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins
    cd00187
    Location:7111174
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes ...
    cd03481
    Location:265417
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:251497
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam08070
    Location:14351523
    DTHCT; DTHCT (NUC029) region

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38 Primary Assembly

Genomic

  1. NC_000017.11 

    Range
    40388521..40417950
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005257632.1XP_005257689.1  

    Conserved Domains (6) summary
    cd03365
    Location:443563
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00075
    Location:69170
    HATPase_c; Histidine kinase-like ATPases; This family includes several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins
    cd00187
    Location:6991162
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes ...
    cd03481
    Location:253405
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:131485
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam08070
    Location:14231511
    DTHCT; DTHCT (NUC029) region

Alternate HuRef

Genomic

  1. AC_000149.1 

    Range
    34337633..34367123
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.1

Genomic

  1. NC_018928.2 

    Range
    38780085..38809522
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)